RRC ID 70539
Author Ortmannová J, Sekereš J, Kulich I, Šantrůček J, Dobrev P, Žárský V, Pečenková T.
Title Arabidopsis EXO70B2 exocyst subunit contributes to papillae and encasement formation in antifungal defence.
Journal J Exp Bot
Abstract In the reaction to non-adapted Blumeria graminis f. sp. hordei (Bgh), Arabidopsis thaliana leaf epidermal cells deposit cell wall reinforcements called papillae or seal fungal haustoria in encasements, both of which involve intensive exocytosis. A plant syntaxin, SYP121/PEN1, has been found to be of key importance for the timely formation of papillae, and the vesicle tethering complex exocyst subunit EXO70B2 has been found to contribute to their morphology. Here, we identify a specific role for the EXO70B2-containing exocyst complex in the papillae membrane domains important for callose deposition and GFP-SYP121 delivery to the focal attack sites, as well as its contribution to encasement formation. The mRuby2-EXO70B2 co-localizes with the exocyst core subunit SEC6 and GFP-SYP121 in the membrane domain of papillae, and EXO70B2 and SYP121 proteins have the capacity to directly interact. The exo70B2/syp121 double mutant produces a reduced number of papillae and haustorial encasements in response to Bgh, indicating an additive role of the exocyst in SYP121-coordinated non-host resistance. In summary, we report cooperation between the plant exocyst and a SNARE protein in penetration resistance against non-adapted fungal pathogens.
Volume 73(3)
Pages 742-755
Published 2022-1-27
DOI 10.1093/jxb/erab457
PII 6402053
PMID 34664667
MeSH Arabidopsis* / metabolism Arabidopsis Proteins* / metabolism Cell Wall / metabolism Qa-SNARE Proteins / genetics Qa-SNARE Proteins / metabolism Vesicular Transport Proteins
IF 5.908
Arabidopsis / Cultured plant cells, genes pda08020