論文 - 詳細
| RRC ID | 7053 |
|---|---|
| 著者 | Tian J, Wang Y, Dong X. |
| タイトル | Methanoculleus hydrogenitrophicus sp. nov., a methanogenic archaeon isolated from wetland soil. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
An obligately anaerobic, methanogenic archaeon, strain HC(T), was isolated from soil of the Zoige wetland on the Tibetan plateau, China. The strain was isolated through construction of an artificial butyrate-degrading consortium in co-culture with a syntrophic bacterium, 'Syntrophomonas erecta subsp. sporosyntropha' JCM 13344. Cells of strain HC(T) were irregular coccoids, 0.8-2 mum in diameter, that occurred singly and utilized only H(2)/CO(2) for growth and methane production. Growth occurred at 18-45 degrees C (optimum around 37 degrees C). The pH for growth was 5.0-8.5 (optimal growth around pH 6.6). The G+C content of the genomic DNA was 60.2 mol%. 16S rRNA gene sequence analysis indicated that strain HC(T) was affiliated to the genus Methanoculleus, with sequence similarities of 94.8-97.2 % to existing members. However, strain HC(T) was distinguished from described Methanoculleus species by not using formate for growth or methane formation and not requiring acetate as a growth factor. On the basis of phylogenetic analysis and phenotypic characteristics, the novel species Methanoculleus hydrogenitrophicus sp. nov. is proposed, with strain HC(T) (=CGMCC 1.5146(T) =JCM 16311(T)) as the type strain. |
| 巻・号 | 60(Pt 9) |
| ページ | 2165-2169 |
| 公開日 | 2010-9-1 |
| DOI | 10.1099/ijs.0.019273-0 |
| PMID | 19897615 |
| MeSH | DNA, Archaeal / genetics DNA, Ribosomal / genetics Methane / metabolism* Methanomicrobiaceae / classification* Methanomicrobiaceae / genetics Methanomicrobiaceae / isolation & purification* Methanomicrobiaceae / metabolism Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics Soil Microbiology* Wetlands |
| IF | 2.415 |
| 引用数 | 15 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 16311 JCM 13344 |