| RRC ID |
70552
|
| 著者 |
Nordholt N, Kanaris O, Schmidt SBI, Schreiber F.
|
| タイトル |
Persistence against benzalkonium chloride promotes rapid evolution of tolerance during periodic disinfection.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Biocides used as disinfectants are important to prevent the transmission of pathogens, especially during the current antibiotic resistance crisis. This crisis is exacerbated by phenotypically tolerant persister subpopulations that can survive transient antibiotic treatment and facilitate resistance evolution. Here, we show that E. coli displays persistence against a widely used disinfectant, benzalkonium chloride (BAC). Periodic, persister-mediated failure of disinfection rapidly selects for BAC tolerance, which is associated with reduced cell surface charge and mutations in the lpxM locus, encoding an enzyme for lipid A biosynthesis. Moreover, the fitness cost incurred by BAC tolerance turns into a fitness benefit in the presence of antibiotics, suggesting a selective advantage of BAC-tolerant mutants in antibiotic environments. Our findings highlight the links between persistence to disinfectants and resistance evolution to antimicrobials.
|
| 巻・号 |
12(1)
|
| ページ |
6792
|
| 公開日 |
2021-11-23
|
| DOI |
10.1038/s41467-021-27019-8
|
| PII |
10.1038/s41467-021-27019-8
|
| PMID |
34815390
|
| PMC |
PMC8611074
|
| MeSH |
Acyltransferases
Anti-Bacterial Agents / pharmacology*
Anti-Bacterial Agents / therapeutic use
Benzalkonium Compounds / pharmacology
Disinfectants / pharmacology*
Disinfection / methods
Drug Resistance, Bacterial / genetics*
Escherichia coli / drug effects*
Escherichia coli / genetics
Escherichia coli Proteins
Evolution, Molecular*
Genetic Loci
Microbial Sensitivity Tests
|
| IF |
12.121
|
| リソース情報 |
| 原核生物(大腸菌) |
JW1844-AM
JW2557-AM
JW1844-KC
BW25113 |
