論文 - 詳細
| RRC ID | 70573 |
|---|---|
| 著者 | Böhm UL, Kimura Y, Kawashima T, Ahrens MB, Higashijima SI, Engert F, Cohen AE. |
| タイトル | Voltage imaging identifies spinal circuits that modulate locomotor adaptation in zebrafish. |
| ジャーナル | Neuron |
| Abstract |
Motor systems must continuously adapt their output to maintain a desired trajectory. While the spinal circuits underlying rhythmic locomotion are well described, little is known about how the network modulates its output strength. A major challenge has been the difficulty of recording from spinal neurons during behavior. Here, we use voltage imaging to map the membrane potential of large populations of glutamatergic neurons throughout the spinal cord of the larval zebrafish during fictive swimming in a virtual environment. We characterized a previously undescribed subpopulation of tonic-spiking ventral V3 neurons whose spike rate correlated with swimming strength and bout length. Optogenetic activation of V3 neurons led to stronger swimming and longer bouts but did not affect tail beat frequency. Genetic ablation of V3 neurons led to reduced locomotor adaptation. The power of voltage imaging allowed us to identify V3 neurons as a critical driver of locomotor adaptation in zebrafish. |
| 巻・号 | 110(7) |
| ページ | 1211-1222.e4 |
| 公開日 | 2022-4-6 |
| DOI | 10.1016/j.neuron.2022.01.001 |
| PII | S0896-6273(22)00001-0 |
| PMID | 35104451 |
| PMC | PMC8989672 |
| MeSH | Animals Locomotion / physiology Motor Neurons* / physiology Spinal Cord / physiology Swimming Zebrafish* / physiology |
| IF | 14.415 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 33 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(vglut2a:Gal4) Tg(vglut2a:loxP-DsRed-loxP-ChR-GFP) Tg(nkx2.2b:Cre-mCherry-NLS) Tg(sim1a:loxP-DsRed-loxP-DTA) Tg(hoxa4a/9a:Cre) |