| RRC ID |
7070
|
| Author |
Cui HL, Yang X, Gao X, Li XY, Xu XW, Zhou YG, Liu HC, Zhou PJ.
|
| Title |
Halogeometricum rufum sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halogeometricum.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Two halophilic archaea, strains RO1-4(T) and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4(T) and RO1-64 were able to grow at 25-55 °C (optimum 40-42 °C), at 2.1-5.1 M NaCl (optimum 3.9 M NaCl), at 0.05-0.7 M MgCl(2) (optimum 0.3 M MgCl(2)) and at pH 6.0-8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12 % (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4(T) and RO1-64 were closely related to Halogeometricum borinquense PR3(T) (98.0 and 98.2 % similarity, respectively) and Halosarcina pallida BZ256(T) (97.8 and 97.9 %). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4(T) and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA-DNA hybridization value between strains RO1-4(T) and RO1-64 was 83.0 % and both strains showed low DNA-DNA relatedness with Halogeometricum borinquense PR3(T) (42.5 and 50.1 % relatedness, respectively) and Halosarcina pallida BZ256(T) (37.6 and 42.1 % relatedness). It was concluded that strains RO1-4(T) and RO1-64 represent a novel species of the genus Halogeometricum, for which the name Halogeometricum rufum sp. nov. is proposed. The type strain is RO1-4(T) (=CGMCC 1.7736(T) =JCM 15770(T)).
|
| Volume |
60(Pt 11)
|
| Pages |
2613-2617
|
| Published |
2010-11-1
|
| DOI |
10.1099/ijs.0.019463-0
|
| PMID |
20023063
|
| MeSH |
DNA, Archaeal / genetics
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification*
Halobacteriaceae / metabolism
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sodium Chloride / metabolism*
|
| IF |
2.415
|
| Times Cited |
9
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 15770
JCM 14791
JCM 10706 |
