| RRC ID |
7073
|
| 著者 |
Cui HL, Gao X, Li XY, Xu XW, Zhou YG, Liu HC, Zhou PJ.
|
| タイトル |
Halosarcina limi sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halosarcina.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A halophilic archaeon, strain RO1-6(T), was isolated from a marine solar saltern in eastern China. Cells of strain RO1-6(T) were pleomorphic and motile and stained Gram-negative. Strain RO1-6(T) grew well on complex medium and colonies were red-pigmented. It was able to grow at 20-50°C (optimum 37°C), in 2.1-5.1M NaCl (optimum 3.9 M NaCl), in 0.05-0.70 M MgCl₂ (optimum 0.30 M MgCl₂) and at pH 6.5-8.0 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO1-6(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids that were chromatographically identical to S-DGD-1 and S₂-DGD. The 16S rRNA gene sequence of strain RO1-6(T) showed similarities of 96.9 and 96.4 % to those of the type strains of Halosarcina pallida and Halogeometricum borinquense, respectively, members of the most closely related recognized genera within the family Halobacteriaceae. The DNA G+C content of strain RO1-6(T) was 61.2 mol%. Phenotypic characterization and phylogenetic analysis revealed that strain RO1-6(T) is related to Halosarcina pallida and represents a novel species of the genus Halosarcina, for which the name Halosarcina limi sp. nov. is proposed; the type strain is RO1-6(T) (=CGMCC 1.8711(T) =JCM 16054(T)).
|
| 巻・号 |
60(Pt 10)
|
| ページ |
2462-3466
|
| 公開日 |
2010-10-1
|
| DOI |
10.1099/ijs.0.018697-0
|
| PMID |
19946053
|
| MeSH |
Base Composition
China
Cluster Analysis
DNA, Archaeal / chemistry
DNA, Archaeal / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Genes, rRNA
Geologic Sediments / microbiology*
Glycolipids / analysis
Halobacteriaceae / classification*
Halobacteriaceae / genetics
Halobacteriaceae / isolation & purification*
Halobacteriaceae / physiology
Hydrogen-Ion Concentration
Locomotion
Magnesium Chloride / metabolism
Molecular Sequence Data
Phospholipids / analysis
Phylogeny
Pigments, Biological / biosynthesis
RNA, Archaeal / genetics
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Sodium Chloride / metabolism
Temperature
|
| IF |
2.415
|
| 引用数 |
8
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 16054
JCM 13897
JCM 10706 |
