RRC ID 70760
Author Shin D, Nakamura M, Morishita Y, Eiraku M, Yamakawa T, Sasamura T, Akiyama M, Inaki M, Matsuno K.
Title Collective nuclear behavior shapes bilateral nuclear symmetry for subsequent left-right asymmetric morphogenesis in Drosophila.
Journal Development
Abstract Proper organ development often requires nuclei to move to a specific position within the cell. To determine how nuclear positioning affects left-right (LR) development in the Drosophila anterior midgut (AMG), we developed a surface-modeling method to measure and describe nuclear behavior at stages 13-14, captured in three-dimensional time-lapse movies. We describe the distinctive positioning and a novel collective nuclear behavior by which nuclei align LR symmetrically along the anterior-posterior axis in the visceral muscles that overlie the midgut and are responsible for the LR-asymmetric development of this organ. Wnt4 signaling is crucial for the collective behavior and proper positioning of the nuclei, as are myosin II and the LINC complex, without which the nuclei fail to align LR symmetrically. The LR-symmetric positioning of the nuclei is important for the subsequent LR-asymmetric development of the AMG. We propose that the bilaterally symmetrical positioning of these nuclei may be mechanically coupled with subsequent LR-asymmetric morphogenesis.
Volume 148(18)
Published 2021-9-15
DOI 10.1242/dev.198507
PII 260539
PMID 34097729
PMC PMC8126412
MeSH Animals Body Patterning / physiology* Cell Nucleus / metabolism Cell Nucleus / physiology* Digestive System / metabolism Digestive System / physiopathology* Drosophila / metabolism Drosophila / physiology* Drosophila Proteins / metabolism Gene Expression Regulation, Developmental / physiology Morphogenesis / physiology* Muscles / metabolism Muscles / physiology Myosin Type II / metabolism Signal Transduction / physiology
IF 5.611
Drosophila DGRC#113545