論文 - 詳細
| RRC ID | 70763 |
|---|---|
| 著者 | Yang K, Liu M, Feng Z, Rojas M, Zhou L, Ke H, Pastor-Pareja JC. |
| タイトル | ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriers. |
| ジャーナル | Cell Rep |
| Abstract |
Secretory cargos are collected at endoplasmic reticulum (ER) exit sites (ERES) before transport to the Golgi apparatus. Decades of research have provided many details of the molecular events underlying ER-Golgi exchanges. Essential questions, however, remain about the organization of the ER-Golgi interface in cells and the type of membrane structures mediating traffic from ERES. To investigate these, we use transgenic tagging in Drosophila flies, 3D-structured illumination microscopy (SIM), and focused ion beam scanning electron microscopy (FIB-SEM) to characterize ERES-Golgi units in collagen-producing fat body, imaginal discs, and imaginal discs overexpressing ERES determinant Tango1. Facing ERES, we find a pre-cis-Golgi region, equivalent to the vertebrate ER-Golgi intermediate compartment (ERGIC), involved in both anterograde and retrograde transport. This pre-cis-Golgi is continuous with the rest of the Golgi, not a separate compartment or collection of large carriers, for which we find no evidence. We observe, however, many vesicles, as well as pearled tubules connecting ERES and Golgi. |
| 巻・号 | 36(11) |
| ページ | 109707 |
| 公開日 | 2021-9-14 |
| DOI | 10.1016/j.celrep.2021.109707 |
| PII | S2211-1247(21)01154-2 |
| PMID | 34525362 |
| MeSH | ADP-Ribosylation Factor 1 / metabolism Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / metabolism Aryl Hydrocarbon Receptor Nuclear Translocator / metabolism Biological Transport COP-Coated Vesicles / metabolism* Drosophila / metabolism* Drosophila Proteins / metabolism Endoplasmic Reticulum / chemistry Endoplasmic Reticulum / metabolism* Golgi Apparatus / chemistry Golgi Apparatus / metabolism* Golgi Matrix Proteins / metabolism Microscopy, Electron, Scanning Monomeric GTP-Binding Proteins / metabolism |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 26 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109702 |