Reference - Detail
| RRC ID | 70770 |
|---|---|
| Author | di Pietro F, Herszterg S, Huang A, Bosveld F, Alexandre C, Sancéré L, Pelletier S, Joudat A, Kapoor V, Vincent JP, Bellaïche Y. |
| Title | Rapid and robust optogenetic control of gene expression in Drosophila. |
| Journal | Dev Cell |
| Abstract |
Deciphering gene function requires the ability to control gene expression in space and time. Binary systems such as the Gal4/UAS provide a powerful means to modulate gene expression and to induce loss or gain of function. This is best exemplified in Drosophila, where the Gal4/UAS system has been critical to discover conserved mechanisms in development, physiology, neurobiology, and metabolism, to cite a few. Here we describe a transgenic light-inducible Gal4/UAS system (ShineGal4/UAS) based on Magnet photoswitches. We show that it allows efficient, rapid, and robust activation of UAS-driven transgenes in different tissues and at various developmental stages in Drosophila. Furthermore, we illustrate how ShineGal4 enables the generation of gain and loss-of-function phenotypes at animal, organ, and cellular levels. Thanks to the large repertoire of UAS-driven transgenes, ShineGal4 enriches the Drosophila genetic toolkit by allowing in vivo control of gene expression with high temporal and spatial resolutions. |
| Volume | 56(24) |
| Pages | 3393-3404.e7 |
| Published | 2021-12-20 |
| DOI | 10.1016/j.devcel.2021.11.016 |
| PII | S1534-5807(21)00940-0 |
| PMID | 34879263 |
| PMC | PMC8693864 |
| MeSH | Animals Body Patterning / genetics Body Patterning / radiation effects Drosophila melanogaster / genetics* Drosophila melanogaster / radiation effects Gene Expression Regulation, Developmental* / radiation effects Light Optogenetics* Organ Specificity / genetics Organ Specificity / radiation effects Pupa / genetics Pupa / radiation effects Time Factors |
| IF | 10.092 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 266 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#106343 |