RRC ID |
7078
|
著者 |
Iino T, Mori K, Suzuki KI.
|
タイトル |
Methanospirillum lacunae sp. nov., a methane-producing archaeon isolated from a puddly soil, and emended descriptions of the genus Methanospirillum and Methanospirillum hungatei.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
A mesophilic, hydrogenotrophic methanogen, designated strain Ki8-1(T), was isolated from soil. Cells were strictly anaerobic, Gram-stain-negative, non-sporulating, motile by means of a single flagellum or tufted flagella, and curved or wavy rod-shaped (11-25 μm long). The temperature and pH for optimum growth were 30 °C and 7.5. The strain grew best in basal medium without the addition of NaCl. Methane was produced from H(2) and formate. Acetate or yeast extract was required for growth. The G+C content of the genomic DNA of strain Ki8-1(T) was 45.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Ki8-1(T) was a member of the genus Methanospirillum and showed 95.1% sequence similarity to Methanospirillum hungatei NBRC 100397(T). On the basis of its phenotypic characteristics and phylogenetic position, strain Ki8-1(T) is considered to represent a novel species of the genus Methanospirillum, for which the name Methanospirillum lacunae sp. nov. is proposed. The type strain is Ki8-1(T) (NBRC 104920(T) =JCM 16384(T) =DSM 22751(T)). Emended descriptions of the genus Methanospirillum and of Methanospirillum hungatei are also provided.
|
巻・号 |
60(Pt 11)
|
ページ |
2563-2566
|
公開日 |
2010-11-1
|
DOI |
10.1099/ijs.0.020131-0
|
PMID |
20008107
|
MeSH |
Base Composition
DNA, Archaeal / genetics
Methane / metabolism*
Methanospirillum / classification*
Methanospirillum / genetics
Methanospirillum / isolation & purification*
Methanospirillum / metabolism
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
Soil Microbiology*
|
IF |
2.415
|
引用数 |
34
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 16384 |