| RRC ID |
70787
|
| 著者 |
Yang S, Wu X, Daoutidou EI, Zhang Y, Shimell M, Chuang KH, Peterson AJ, O'Connor MB, Zheng X.
|
| タイトル |
The NDNF-like factor Nord is a Hedgehog-induced extracellular BMP modulator that regulates Drosophila wing patterning and growth.
|
| ジャーナル |
Elife
|
| Abstract |
Hedgehog (Hh) and Bone Morphogenetic Proteins (BMPs) pattern the developing Drosophila wing by functioning as short- and long-range morphogens, respectively. Here, we show that a previously unknown Hh-dependent mechanism fine-tunes the activity of BMPs. Through genome-wide expression profiling of the Drosophila wing imaginal discs, we identify nord as a novel target gene of the Hh signaling pathway. Nord is related to the vertebrate Neuron-Derived Neurotrophic Factor (NDNF) involved in congenital hypogonadotropic hypogonadism and several types of cancer. Loss- and gain-of-function analyses implicate Nord in the regulation of wing growth and proper crossvein patterning. At the molecular level, we present biochemical evidence that Nord is a secreted BMP-binding protein and localizes to the extracellular matrix. Nord binds to Decapentaplegic (Dpp) or the heterodimer Dpp-Glass-bottom boat (Gbb) to modulate their release and activity. Furthermore, we demonstrate that Nord is a dosage-dependent BMP modulator, where low levels of Nord promote and high levels inhibit BMP signaling. Taken together, we propose that Hh-induced Nord expression fine-tunes both the range and strength of BMP signaling in the developing Drosophila wing.
|
| 巻・号 |
11
|
| 公開日 |
2022-1-17
|
| DOI |
10.7554/eLife.73357
|
| PII |
73357
|
| PMID |
35037619
|
| PMC |
PMC8856659
|
| MeSH |
Animals
Body Patterning / genetics
Body Patterning / physiology*
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster / growth & development*
Gene Expression Regulation, Developmental / physiology*
Hedgehog Proteins / genetics
Hedgehog Proteins / metabolism*
Larva / growth & development
Larva / metabolism
Wings, Animal / growth & development*
|
| IF |
7.08
|
| リソース情報 |
| ショウジョウバエ |
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