Reference - Detail
| RRC ID | 70818 |
|---|---|
| Author | Girard JR, Goins LM, Vuu DM, Sharpley MS, Spratford CM, Mantri SR, Banerjee U. |
| Title | Paths and pathways that generate cell-type heterogeneity and developmental progression in hematopoiesis. |
| Journal | Elife |
| Abstract |
Mechanistic studies of Drosophila lymph gland hematopoiesis are limited by the availability of cell-type-specific markers. Using a combination of bulk RNA-Seq of FACS-sorted cells, single-cell RNA-Seq, and genetic dissection, we identify new blood cell subpopulations along a developmental trajectory with multiple paths to mature cell types. This provides functional insights into key developmental processes and signaling pathways. We highlight metabolism as a driver of development, show that graded Pointed expression allows distinct roles in successive developmental steps, and that mature crystal cells specifically express an alternate isoform of Hypoxia-inducible factor (Hif/Sima). Mechanistically, the Musashi-regulated protein Numb facilitates Sima-dependent non-canonical, and inhibits canonical, Notch signaling. Broadly, we find that prior to making a fate choice, a progenitor selects between alternative, biologically relevant, transitory states allowing smooth transitions reflective of combinatorial expressions rather than stepwise binary decisions. Increasingly, this view is gaining support in mammalian hematopoiesis. |
| Volume | 10 |
| Published | 2021-10-29 |
| DOI | 10.7554/eLife.67516 |
| PII | 67516 |
| PMID | 34713801 |
| PMC | PMC8610493 |
| MeSH | Animals DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Female Hematopoiesis* Hemocytes / metabolism* Hemolymph / metabolism* Juvenile Hormones / genetics* Juvenile Hormones / metabolism Larva / genetics Larva / growth & development Male |
| IF | 7.08 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#114255 |