RRC ID 70831
著者 Pogodalla N, Kranenburg H, Rey S, Rodrigues S, Cardona A, Klämbt C.
タイトル Drosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil.
ジャーナル Nat Commun
Abstract In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ßHeavy-Spectrin cytoskeleton. ßHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function.
巻・号 12(1)
ページ 6357
公開日 2021-11-4
DOI 10.1038/s41467-021-26462-x
PII 10.1038/s41467-021-26462-x
PMID 34737284
PMC PMC8569210
MeSH Animals Brain / metabolism* Cell Lineage Drosophila Drosophila Proteins / metabolism* Glycoproteins / metabolism Larva Nerve Tissue Proteins / metabolism Neuroglia / metabolism* Neurons / metabolism* Neuropil / metabolism* Phosphatidylinositol Phosphates / metabolism Spectrin / metabolism*
IF 12.121
リソース情報
ショウジョウバエ DGRC#108191