RRC ID 70854
Author Sainz de la Maza D, Hof-Michel S, Phillimore L, Bökel C, Amoyel M.
Title Cell-cycle exit and stem cell differentiation are coupled through regulation of mitochondrial activity in the Drosophila testis.
Journal Cell Rep
Abstract Whereas stem and progenitor cells proliferate to maintain tissue homeostasis, fully differentiated cells exit the cell cycle. How cell identity and cell-cycle state are coordinated during differentiation is still poorly understood. The Drosophila testis niche supports germline stem cells and somatic cyst stem cells (CySCs). CySCs give rise to post-mitotic cyst cells, providing a tractable model to study the links between stem cell identity and proliferation. We show that, while cell-cycle progression is required for CySC self-renewal, the E2f1/Dp transcription factor is dispensable for self-renewal but instead must be silenced by the Drosophila retinoblastoma homolog, Rbf, to permit differentiation. Continued E2f1/Dp activity inhibits the expression of genes important for mitochondrial activity. Furthermore, promoting mitochondrial biogenesis rescues the differentiation of CySCs with ectopic E2f1/Dp activity but not their cell-cycle exit. In sum, E2f1/Dp coordinates cell-cycle progression with stem cell identity by regulating the metabolic state of CySCs.
Volume 39(6)
Pages 110774
Published 2022-5-10
DOI 10.1016/j.celrep.2022.110774
PII S2211-1247(22)00538-1
PMID 35545055
PMC PMC9350557
MeSH Animals Cell Cycle Cell Differentiation / genetics Cysts* / metabolism Drosophila / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / metabolism Male Retinoblastoma Protein / metabolism Stem Cell Niche / genetics Testis Transcription Factors / metabolism
IF 8.109
Drosophila DGRC#115279