RRC ID 70876
Author Habicher J, Manuel R, Pedroni A, Ferebee C, Ampatzis K, Boije H.
Title A new transgenic reporter line reveals expression of protocadherin 9 at a cellular level within the zebrafish central nervous system.
Journal Gene Expr Patterns
Abstract The wiring of neuronal networks is far from understood. One outstanding question is how neurons of different types link up to form subnetworks within the greater context. Cadherins have been suggested to create an inclusion code where interconnected neurons express the same subtypes. Here, we have used a CRISPR/Cas9 knock-in approach to generate a transgenic zebrafish reporter line for protocadherin 9 (pcdh9), which is predominantly expressed within the central nervous system. Expression of eGFP was detected in subsets of neurons in the cerebellum, retina and spinal cord, in both larvae and juveniles. A closer characterization of the spinal locomotor network revealed that a portion of distinct classes of both excitatory and inhibitory interneurons, as well as motor neurons, expressed pcdh9. This transgenic line could thus be used to test the cadherin network hypothesis, through electrophysiological characterization of eGFP positive cells, to show if these are synaptically connected and form a discrete network within the spinal cord.
Volume 44
Pages 119246
Published 2022-6-1
DOI 10.1016/j.gep.2022.119246
PII S1567-133X(22)00016-3
PMID 35427788
MeSH Animals Animals, Genetically Modified Cadherins / genetics Cadherins / metabolism Central Nervous System / metabolism Motor Neurons / metabolism Protocadherins* Spinal Cord / metabolism Zebrafish* / genetics Zebrafish* / metabolism
IF 0.897
Zebrafish Tg(dmrt3a:Gal4) UAS:RFP