論文 - 詳細
| RRC ID | 70880 |
|---|---|
| 著者 | Antón-Galindo E, Dalla Vecchia E, Orlandi JG, Castro G, Gualda EJ, Young AMJ, Guasch-Piqueras M, Arenas C, Herrera-Úbeda C, Garcia-Fernàndez J, Aguado F, Loza-Alvarez P, Cormand B, Norton WHJ, Fernàndez-Castillo N. |
| タイトル | Deficiency of the ywhaz gene, involved in neurodevelopmental disorders, alters brain activity and behaviour in zebrafish. |
| ジャーナル | Mol Psychiatry |
| Abstract |
Genetic variants in YWHAZ contribute to psychiatric disorders such as autism spectrum disorder and schizophrenia, and have been related to an impaired neurodevelopment in humans and mice. Here, we have used zebrafish to investigate the mechanisms by which YWHAZ contributes to neurodevelopmental disorders. We observed that ywhaz expression was pan-neuronal during developmental stages and restricted to Purkinje cells in the adult cerebellum, cells that are described to be reduced in number and size in autistic patients. We then performed whole-brain imaging in wild-type and ywhaz CRISPR/Cas9 knockout (KO) larvae and found altered neuronal activity and connectivity in the hindbrain. Adult ywhaz KO fish display decreased levels of monoamines in the hindbrain and freeze when exposed to novel stimuli, a phenotype that can be reversed with drugs that target monoamine neurotransmission. These findings suggest an important role for ywhaz in establishing neuronal connectivity during development and modulating both neurotransmission and behaviour in adults. |
| 巻・号 | 27(9) |
| ページ | 3739-3748 |
| 公開日 | 2022-9-1 |
| DOI | 10.1038/s41380-022-01577-9 |
| PII | 10.1038/s41380-022-01577-9 |
| PMID | 35501409 |
| MeSH | 14-3-3 Proteins* / genetics Animals Autism Spectrum Disorder / genetics Autism Spectrum Disorder / physiopathology Autistic Disorder / genetics Autistic Disorder / physiopathology Brain* / metabolism Brain* / physiopathology Humans Neurodevelopmental Disorders / genetics Neurodevelopmental Disorders / physiopathology Zebrafish* / genetics Zebrafish Proteins* / genetics |
| IF | 12.384 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(aldoca:gap43-Venus)rk22Tg |