論文 - 詳細
| RRC ID | 70913 |
|---|---|
| 著者 | Liu J, Bao Y, Zhang X, Zhang K, Chen S, Wu H, He J. |
| タイトル | Proteiniclasticum sediminis sp. nov., an obligate anaerobic bacterium isolated from anaerobic sludge. |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
An obligate anaerobic bacterial BAD-10 T was isolated from anaerobic acetochlor-degrading sludge. The strain was Gram-stain negative, curved rod-shaped, non-motile and non-spore-forming. Growth was observed in PYT medium at pH 6.0-9.0 (optimum, pH 7.5), at 25-47 °C (37 °C) and with 0-1.0% NaCl (w/v, 0%). Strain BAD-10 T could degrade acetochlor. The major fermentation products from peptone-yeast (PY) medium were acetate and butyrate. The predominant cellular fatty acids were iso-C15:0 FAME, anteiso-C15:0 FAME and C16:0 FAME. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain BAD-10 T showed closest affiliation to Proteiniclasticum ruminis D3RC-2 T, with a sequence similarity of 97.6%. Genome sequencing revealed a genome size of 2,983,986 bp, a G + C content of 51.4 mol% and protein-coding genes of 3,102. The average nucleotide identity and in silico DNA-DNA hybridization values between strain BAD-10 T and Proteiniclasticum ruminis D3RC-2 T were 71.0% and 20.4%, respectively, which were below the standard thresholds for species differentiation. On the basis of phenotypic, physiological and phylogenetic evidence, strain BAD-10 T represents a novel species in the genus Proteiniclasticum, for which the name Proteiniclasticum sediminis sp. nov. is proposed. Strain BAD-10 T (= CCTCC AB 2021091 T = KCTC 25288 T) is the type strain of the proposed novel species. |
| 巻・号 | 114(10) |
| ページ | 1541-1549 |
| 公開日 | 2021-10-1 |
| DOI | 10.1007/s10482-021-01620-9 |
| PII | 10.1007/s10482-021-01620-9 |
| PMID | 34401954 |
| MeSH | Anaerobiosis Bacterial Typing Techniques Base Composition Clostridiaceae DNA, Bacterial / genetics Fatty Acids* Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sewage* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM14817 |