| RRC ID |
70917
|
| Author |
Maeng S, Park Y, Han JH, Lee SE, Kim MK, Subramani G.
|
| Title |
Pseudocnuella soli gen. nov., sp. nov., a bacterium from soil belonging to the family Chitinophagaceae.
|
| Journal |
Antonie Van Leeuwenhoek
|
| Abstract |
A Gram-stain negative, aerobic, rod-shaped, yellow-coloured bacterium, designated strain 17J28-1T, was isolated from soil of Jeju Island, Korea. Optimal growth was found to occur at 30 °C, at pH 6.0 and in the absence of NaCl on R2A at 30 °C. Strain 17J28-1T showed resistance to UV-radiation. The draft genome sequence of strain 17J28-1T is 4804,510 bp long with a 49.4% G + C content. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 17J28-1T forms a distinct lineage within the family Chitinophagaceae (order Chitinophagales, class Chitinophagia) and is closely related to Cnuella takakiae (93.7% 16S rRNA gene sequence similarity) and Flavisolibacter ginsengiterrae (93.1%). In addition, genomic comparison using digital DNA-DNA hybridization (dDDH) and OrthoANI analyses between the novel organism and the members of the family Chitinophagaceae revealed identities of 65.5-74.1% and 18.1-28%, respectively. The predominant cellular fatty acids were identified as C15:0 iso, C15:1 iso G and C17:0 iso 3-OH and the major respiratory quinone as MK-7. Based on these data we propose that strain 17J28-1T (= KCTC 62223T = JCM 33202T) represents a novel genus and species in the family Chitinophagaceae, for which the name Pseudocnuella soli gen. nov. sp. nov. is proposed.
|
| Volume |
114(2)
|
| Pages |
161-168
|
| Published |
2021-2-1
|
| DOI |
10.1007/s10482-020-01509-z
|
| PII |
10.1007/s10482-020-01509-z
|
| PMID |
33449221
|
| MeSH |
Bacterial Typing Techniques
Bacteroidetes
DNA, Bacterial / genetics
Fatty Acids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil*
Soil Microbiology*
|
| Resource |
| General Microbes |
JCM33202 |
