| RRC ID |
70920
|
| Author |
Huang RR, Yang SR, Zhen C, Ge XF, Chen XK, Wen ZQ, Li YN, Liu WZ.
|
| Title |
Genomic molecular signatures determined characterization of Mycolicibacterium gossypii sp. nov., a fast-growing mycobacterial species isolated from cotton field soil.
|
| Journal |
Antonie Van Leeuwenhoek
|
| Abstract |
A Gram-positive, acid-fast and rapidly growing rod, designated S2-37 T, that could form yellowish colonies was isolated from one soil sample collected from cotton cropping field located in the Xinjiang region of China. Genomic analyses indicated that strain S2-37 T harbored T7SS secretion system and was very likely able to produce mycolic acid, which were typical features of pathogenetic mycobacterial species. 16S rRNA-directed phylogenetic analysis referred that strain S2-37 T was closely related to bacterial species belonging to the genus Mycolicibacterium, which was further confirmed by pan-genome phylogenetic analysis. Digital DNA-DNA hybridization and the average nucleotide identity presented that strain S2-37 T displayed the highest values of 39.1% (35.7-42.6%) and 81.28% with M. litorale CGMCC 4.5724 T, respectively. And characterization of conserved molecular signatures further supported the taxonomic position of strain S2-37 T belonging to the genus Mycolicibacterium. The main fatty acids were identified as C16:0, C18:0, C20:3ω3 and C22:6ω3. In addition, polar lipids profile was mainly composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Phylogenetic analyses, distinct fatty aids and antimicrobial resistance profiles indicated that strain S2-37 T represented genetically and phenotypically distinct from its closest phylogenetic neighbour, M. litorale CGMCC 4.5724 T. Here, we propose a novel species of the genus Mycolicibacterium: Mycolicibacterium gossypii sp. nov. with the type strain S2-37 T (= JCM 34327 T = CGMCC 1.18817 T).
|
| Volume |
114(10)
|
| Pages |
1735-1744
|
| Published |
2021-10-1
|
| DOI |
10.1007/s10482-021-01638-z
|
| PII |
10.1007/s10482-021-01638-z
|
| PMID |
34392432
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / analysis
Genomics
Mycobacterium*
Phospholipids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil*
Soil Microbiology
|
| Resource |
| General Microbes |
JCM34327 |
