RRC ID 70921
著者 Pang Y, Zhang Y, Chen M, Lu W, Chen M, Yan Y, Lin M, Zhang W, Zhou Z.
タイトル Pseudomonas nanhaiensis sp. nov., a lipase-producing bacterium isolated from deep-sea sediment of the South China Sea.
ジャーナル Antonie Van Leeuwenhoek
Abstract A bacterial lipase producing bacterium, designated SCS 2-3, was isolated from deep-sea sediment of the South China Sea. Phylogenetic analysis based on the 16S rRNA sequence revealed that strain SCS2-3 belonged to the genus Pseudomonas and had 98.56% similarity to P. xinjiangensis NRRL B-51270T as the closest relative strain. MLSA using four protein-coding genes (dnaK, gyrA, recA, and rpoB) showed strain SCS 2-3 to form a separate branch. ANI and in silico DDH values between strain SCS 2-3 and related type strains of Pseudomonas were less than 81.51% and 23.80%, respectively. Genome comparison showed that strain SCS 2-3 shared 1875 core gene families with other eight closely related type strains in Pseudomonas, and the number of strain-unique genes was 263. Through gene annotations, genes related to lipase were found in the genome. Furthermore, a combination of phenotypic, chemotaxonomic, phylogenetic and genotypic data clearly indicated that strain SCS 2-3 represents a novel species of the genus Pseudomonas, for which the name Pseudomonas nanhaiensis sp. nov. is proposed. The type strain is SCS 2-3T (= GDMCC 1.2219T = JCM 34440T).
巻・号 114(11)
ページ 1791-1804
公開日 2021-11-1
DOI 10.1007/s10482-021-01639-y
PII 10.1007/s10482-021-01639-y
PMID 34392431
MeSH Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Fatty Acids / analysis Humans Lipase* / genetics Nucleic Acid Hybridization Phylogeny Pseudomonas* / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
リソース情報
一般微生物 JCM34440 JCM14963