| RRC ID |
70938
|
| Author |
Iino T, Oshima K, Hattori M, Ohkuma M, Amachi S.
|
| Title |
Iodidimonas gelatinilytica sp. nov., aerobic iodide-oxidizing bacteria isolated from brine water and surface seawater.
|
| Journal |
Antonie Van Leeuwenhoek
|
| Abstract |
Chemo-organotrophic iodide (I-)-oxidizing bacterial strains Hi-2T and Mie-1 were isolated from iodide-rich natural gas brine water in Chiba and surface seawater in Mie, Japan, respectively. Cells of strains Hi-2T and Mie-1 were aerobic, Gram-negative and rod-shaped (0.3-0.5 µm width and 1.2-4.4 µm in length). Two isolates grew optimally at 30 °C, pH 7.5 and with 3% NaCl (w/v). Iodide oxidation to form molecular iodine (I2) was a biochemically unique trait for strains Hi-2T and Mie-1. The major cellular fatty acids are C18:1ω7c, C16:1ω5c and C18:1 2-OH. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that strains Hi-2T and Mie-1 were located near Iodidimonas muriae C-3T with 99.2% sequence similarity. The calculated digital DNA-DNA hybridization (dDDH) value of 65.7-65.9% between the two isolates and I. muriae C-3T was lower than the threshold of 70%, which was used for prokaryotic species delineation. Strains Hi-2T and Mie-1 differed in the hydrolysis of aesculin, the hydrolysis of gelatin and the major cellular fatty acids composition from I. muriae C-3T. Considering these biochemical properties, the major cellular fatty acids composition and dDDH value, a novel species is proposed for strains Hi-2T (= JCM 17844T = LMG 28661T) and Mie-1 (= JCM 17845 = LMG 28662), to be named Iodidimonas gelatinilytica.
|
| Volume |
114(5)
|
| Pages |
625-631
|
| Published |
2021-5-1
|
| DOI |
10.1007/s10482-021-01546-2
|
| PII |
10.1007/s10482-021-01546-2
|
| PMID |
33761033
|
| MeSH |
Alphaproteobacteria
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids
Iodides*
Nucleic Acid Hybridization
Oxidation-Reduction
Phylogeny
RNA, Ribosomal, 16S / genetics
Salts
Seawater
Sequence Analysis, DNA
Water*
|
| Resource |
| General Microbes |
JCM17844
JCM17845
JCM17843 |
