| RRC ID |
70939
|
| 著者 |
Sun X, Qiu S, Luo X, Jin P, Zhao J, Wu X, Yang J, Wang X, Song J, Xiang W.
|
| タイトル |
Micromonospora rubida sp. nov., a novel actinobacterium isolated from soil of Harbin.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
A novel actinobacterium, designated strain NEAU-HG-1T, was isolated from soil collected from Harbin, Heilongjiang Province, Northeast China and characterised using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-HG-1T belonged to the genus Micromonospora, and shared high sequence similarities with Micromonospora auratinigra DSM 44815T (98.9%) and Micromonospora coerulea DSM 43143T (98.7%). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus Micromonospora. Cell wall contained meso-diaminopimelic acid and the whole-cell sugars were arabinose and xylose. The polar lipid contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipid and phosphatidylinositol. The predominant menaquinones were MK-10(H2), MK-10(H4) and MK-10(H6). The major fatty acids were C17:0 cycle, iso-C15:0, and iso-C16:0. Furthermore, strain NEAU-HG-1T displayed a DNA-DNA relatedness of 33.8 ± 2.2% with M. coerulea DSM 43143T. The level of digital DNA-DNA hybridization between strain NEAU-HG-1T and M. auratinigra DSM 44815T was 27.2% (24.8-29.7%). The value was well below the criteria for species delineation of 70% for dDDH. Whole-genome average nucleotide identity analyses result also indicated that the isolate should be assigned to a new species under the genus Micromonospora. Therefore, it is concluded that strain NEAU-HG-1T represents a novel species of the genus Micromonospora, for which the name Micromonospora rubida sp. nov. is proposed, with NEAU-HG-1T (= CGMCC 4.7479T = JCM 32386T) as the type strain.
|
| 巻・号 |
114(6)
|
| ページ |
697-708
|
| 公開日 |
2021-6-1
|
| DOI |
10.1007/s10482-021-01550-6
|
| PII |
10.1007/s10482-021-01550-6
|
| PMID |
33666807
|
| MeSH |
Bacterial Typing Techniques
DNA, Bacterial / genetics
Fatty Acids / analysis
Micromonospora* / genetics
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil
Soil Microbiology
Vitamin K 2
|
| リソース情報 |
| 一般微生物 |
JCM32386
JCM7348 |
