論文 - 詳細
| RRC ID | 70945 |
|---|---|
| 著者 | Chen MS, Li FN, Chen XH, Yan XR, Tuo L. |
| タイトル | Brachybacterium halotolerans sp. nov., a halotolerant, endophytic actinomycete isolated from branch of Bruguiera gymnoirhiza. |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
A novel, aerobic, moderately halophilic Gram-positive actinomycete, strain MASK1Z-5T was isolated from a surface-sterilized branch of Bruguiera gymnoirhiza in Shankou Mangrove Nature Reserve, Guangxi, China. The taxonomic position of the strain was investigated using a polyphasic approach. Strain MASK1Z-5T tolerated up to 20% (w/v) NaCl (optimum 0-7%), and grew at pH 5.0-12.0 (optimum pH 7.0-8.0), 20-37 °C (optimum 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MASK1Z-5T belonged to the genus Brachybacterium, and showed the highest 16S rRNA gene sequence similarity of 98.0% to B. endophyticum M1HQ-2T. The cell-wall peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. MK-7 was the predominant menaquinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid and three unidentified lipids. The major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The DNA G + C content was calculated to be 71.8 mol% based on the whole genome sequence. The estimated values of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) based on whole genome sequences between strain MASK1Z-5T and B. endophyticum M1HQ-2T were 81.8% and 25.0%, respectively. The phenotypic, chemotaxonomic and genotypic properties clearly indicated that strain MASK1Z-5T represents a novel species within the genus Brachybacterium, for which the name Brachybacterium halotolerans sp. nov. is proposed. The type strain is MASK1Z-5T (= CGMCC1.18660T = JCM 34339T). |
| 巻・号 | 114(6) |
| ページ | 875-884 |
| 公開日 | 2021-6-1 |
| DOI | 10.1007/s10482-021-01565-z |
| PII | 10.1007/s10482-021-01565-z |
| PMID | 33796984 |
| MeSH | Actinobacteria* / genetics Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Fatty Acids / analysis Phospholipids / analysis Phylogeny RNA, Ribosomal, 16S / genetics Rhizophoraceae* Sequence Analysis, DNA |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM34339 |