| RRC ID |
70945
|
| 著者 |
Chen MS, Li FN, Chen XH, Yan XR, Tuo L.
|
| タイトル |
Brachybacterium halotolerans sp. nov., a halotolerant, endophytic actinomycete isolated from branch of Bruguiera gymnoirhiza.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
A novel, aerobic, moderately halophilic Gram-positive actinomycete, strain MASK1Z-5T was isolated from a surface-sterilized branch of Bruguiera gymnoirhiza in Shankou Mangrove Nature Reserve, Guangxi, China. The taxonomic position of the strain was investigated using a polyphasic approach. Strain MASK1Z-5T tolerated up to 20% (w/v) NaCl (optimum 0-7%), and grew at pH 5.0-12.0 (optimum pH 7.0-8.0), 20-37 °C (optimum 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MASK1Z-5T belonged to the genus Brachybacterium, and showed the highest 16S rRNA gene sequence similarity of 98.0% to B. endophyticum M1HQ-2T. The cell-wall peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. MK-7 was the predominant menaquinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid and three unidentified lipids. The major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The DNA G + C content was calculated to be 71.8 mol% based on the whole genome sequence. The estimated values of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) based on whole genome sequences between strain MASK1Z-5T and B. endophyticum M1HQ-2T were 81.8% and 25.0%, respectively. The phenotypic, chemotaxonomic and genotypic properties clearly indicated that strain MASK1Z-5T represents a novel species within the genus Brachybacterium, for which the name Brachybacterium halotolerans sp. nov. is proposed. The type strain is MASK1Z-5T (= CGMCC1.18660T = JCM 34339T).
|
| 巻・号 |
114(6)
|
| ページ |
875-884
|
| 公開日 |
2021-6-1
|
| DOI |
10.1007/s10482-021-01565-z
|
| PII |
10.1007/s10482-021-01565-z
|
| PMID |
33796984
|
| MeSH |
Actinobacteria* / genetics
Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial / genetics
Fatty Acids / analysis
Phospholipids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Rhizophoraceae*
Sequence Analysis, DNA
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| リソース情報 |
| 一般微生物 |
JCM34339 |
