| RRC ID |
70987
|
| Author |
Tian LS, Jiang GQ, Li R, Ni P, Li CE, Zhang HY, Wang J, Zhang XP, Wang Y, Tang SK.
|
| Title |
Qaidamihabitans albus gen. nov., sp. nov., a new member of the family Pseudonocardiaceae, and transfer of Prauserella shujinwangii to Qaidamihabitans gen. nov. as Qaidamihabitans shujinwangii comb. nov.
|
| Journal |
Arch Microbiol
|
| Abstract |
A novel mycelium-forming actinomycete strain, designated YIM S01255T were isolated from a salt lake. Optimal growth occurred in the presence of 0-5.0% (w/v) NaCl, at pH 7.0-8.0, and at 37 °C. Strain YIM S01255T contained meso-diaminopimelic acid as the diagnostic diamino acid, and glucose, galactose and arabinose as the whole-cell sugars. The major fatty acid (> 5.0%) were iso-C16:0, iso-C16:1H and iso-C15:0. The major menaquinone were MK-9(H4) and MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylinositolmannoside and phosphatidylinositol. The DNA G + C content was 70.7 mol%. The 16S rRNA gene sequence of the strain showed high similarity to members of genera in the family Pseudonocardiaceae with values less than 95.8%, and most closely related to the genus Amycolatopsis. Both of phylogenetic analysis based on 16S rRNA gene sequences and the up-to-date bacterial genome sequences analysis revealed that strains YIM S01255T and Prauserella shujinwangii XJ46T formed a distinct monophyletic clade and was separated from the other members within the family Pseudonocardiaceae. The average nucleotide identity (ANI) values and digital DNA-DNA hybridization (dDDH) between the two strains were 81.0% and 40.6%, respectively. The distinctive polyphasic evidences differentiated YIM S01255T from members of the family Pseudonocardiaceae, so strain YIM S01255T is considered to represent a novel species of a novel genus of the family Pseudonocardiaceae, for which the name Qaidamihabitans albus gen. nov., sp. nov. is proposed. The type strain of genus Qaidamihabitans is YIM S01255T (= KCTC 49476T = CGMCC 4.7684T). Moreover, Prauserella shujinwangii is also proposed to being transferred into the genus Qaidamihabitans as Qaidamihabitans shujinwangii comb. nov. (type strain XJ46T = CGMCC 4.7125T = JCM 19736T).
|
| Volume |
203(9)
|
| Pages |
5363-5371
|
| Published |
2021-11-1
|
| DOI |
10.1007/s00203-021-02512-2
|
| PII |
10.1007/s00203-021-02512-2
|
| PMID |
34386827
|
| MeSH |
Actinobacteria
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids*
Phospholipids*
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Vitamin K 2
|
| Resource |
| General Microbes |
JCM19736 |
