| RRC ID |
71017
|
| Author |
Sueda R, Yoshida K, Onodera M, Fukui T, Yatsunami R, Nakamura S.
|
| Title |
Characterization of a GlgC homolog from extremely halophilic archaeon Haloarcula japonica.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Glycogen synthesis in bacteria is mainly organized by the products of glgB, glgC, and glgA genes comprising the widely known glg operon. On the genome of extremely halophilic archaeon Haloarcula japonica, there was a gene cluster analogous to the bacterial glg operon. In this study, we focused on a GlgC homolog of Ha. japonica, and its recombinant enzyme was prepared and characterized. The enzyme showed highest activity toward GTP and glucose-1-phosphate as substrates in the presence of 2.6 m KCl and predicted to be work as "GDP-glucose pyrophosphorylase" in Ha. japonica.
|
| Volume |
85(6)
|
| Pages |
1441-1447
|
| Published |
2021-5-25
|
| DOI |
10.1093/bbb/zbab050
|
| PII |
6179890
|
| PMID |
33749776
|
| MeSH |
Archaeal Proteins / genetics*
Archaeal Proteins / metabolism
Glycogen / biosynthesis
Guanosine Triphosphate / metabolism
Haloarcula / genetics*
Haloarcula / metabolism
Operon / genetics
Sequence Homology, Nucleic Acid*
|
| Resource |
| General Microbes |
JCM7785 |
