Reference - Detail
| RRC ID | 71020 |
|---|---|
| Author | Yokoi KJ, Tsutsui S, Arakawa GY, Takaba M, Fujii K, Kaneko S. |
| Title | Molecular and biochemical characteristics of inulosucrase InuBK from Alkalihalobacillus krulwichiae JCM 11691. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Information about the inulosucrase of nonlactic acid bacteria is scarce. We found a gene encoding inulosucrase (inuBK) in the genome of the Gram-positive bacterium Alkalihalobacillus krulwichiae JCM 11691. The inuBK open reading frame encoded a protein comprising 456 amino acids. We expressed His-tagged InuBK in culture medium using a Brevibacillus system. The optimal pH and temperature of purified InuBK were 7.0-9.0 and 50-55 °C, respectively. The findings of high-performance anion-exchange chromatography, nuclear magnetic resonance spectroscopy, and high-performance size-exclusion chromatography with multiangle laser light scattering showed that the polysaccharide produced by InuBK was an inulin with a molecular weight of 3806, a polydispersity index (PI) of 1.047, and fructosyl chain lengths with 3-27 degrees of polymerization. The size of InuBK was smaller than commercial inulins, and the PI of the inulin that it produced was lower. |
| Volume | 85(8) |
| Pages | 1830-1838 |
| Published | 2021-7-23 |
| DOI | 10.1093/bbb/zbab094 |
| PII | 6280973 |
| PMID | 34021568 |
| MeSH | Bacillaceae / enzymology* Bacillaceae / genetics Chromatography, High Pressure Liquid / methods Cloning, Molecular Culture Media Genes, Bacterial Hexosyltransferases / genetics Hexosyltransferases / isolation & purification Hexosyltransferases / metabolism* Inulin / biosynthesis Magnetic Resonance Spectroscopy / methods Molecular Weight Phylogeny Temperature |
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| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM11691 |