| RRC ID |
71023
|
| Author |
Unno K, Nakagawa H, Kodani S.
|
| Title |
Heterologous production of new protease inhibitory peptide marinostatin E.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Bicyclic peptides, marinostatins, are protease inhibitors derived from the marine bacterium Algicola sagamiensis. The biosynthetic gene cluster of marinostatin was previously identified, although no heterologous production was reported. In this report, the biosynthetic gene cluster of marinostatin (mstA and mstB) was cloned into the expression vector pET-41a(+). As a result of the coexpression experiment, a new analogous peptide named marinostatin E was successfully produced using Escherichia coli BL21(DE3). The structure of marinostatin E was determined by a combination of chemical treatments and tandem mass spectrometry experiments. Marinostatin E exhibited inhibitory activities against chymotrypsin and subtilisin with an IC50 of 4.0 and 39.6 μm, respectively.
|
| Volume |
85(1)
|
| Pages |
97-102
|
| Published |
2021-1-7
|
| DOI |
10.1093/bbb/zbaa011
|
| PII |
6066716
|
| PMID |
33577650
|
| MeSH |
Gammaproteobacteria / genetics
Genetic Engineering*
Multigene Family / genetics
Peptides, Cyclic / biosynthesis*
Peptides, Cyclic / chemistry
Peptides, Cyclic / genetics
Protease Inhibitors / chemistry
Protease Inhibitors / metabolism*
|
| Resource |
| General Microbes |
JCM11461 |
