Reference - Detail
| RRC ID | 71032 |
|---|---|
| Author | Jin X, He Y, Zhou Y, Chen X, Lee YK, Zhao J, Zhang H, Chen W, Wang G. |
| Title | Lactic acid bacteria that activate immune gene expression in Caenorhabditis elegans can antagonise Campylobacter jejuni infection in nematodes, chickens and mice. |
| Journal | BMC Microbiol |
| Abstract |
BACKGROUND:Campylobacter jejuni is the major micro-bacillary pathogen responsible for human coloenteritis. Lactic acid bacteria (LAB) have been shown to protect against Campylobacter infection. However, LAB with a good ability to inhibit the growth of C. jejuni in vitro are less effective in animals and animal models, and have the disadvantages of high cost, a long cycle, cumbersome operation and insignificant immune response indicators. Caenorhabditis elegans is increasingly used to screen probiotics for their anti-pathogenic properties. However, no research on the use of C. elegans to screen for probiotic candidates antagonistic to C. jejuni has been conducted to date. RESULTS:This study established a lifespan model of C. elegans, enabling the preselection of LAB to counter C. jejuni infection. A potential protective mechanism of LAB was identified. Some distinct LAB species offered a high level of protection to C. elegans against C. jejuni. The LAB strains with a high protection rate reduced the load of C. jejuni in C. elegans. The transcription of antibacterial peptide genes, MAPK and Daf-16 signalling pathway-related genes was elevated using the LAB isolates with a high protection rate. The reliability of the lifespan model of C. elegans was verified using mice and chickens infected with C. jejuni. CONCLUSIONS:The results showed that different LAB had different abilities to protect C. elegans against C. jejuni. C. elegans provides a reliable model for researchers to screen for LAB that are antagonistic to C. jejuni on a large scale. |
| Volume | 21(1) |
| Pages | 169 |
| Published | 2021-6-5 |
| DOI | 10.1186/s12866-021-02226-x |
| PII | 10.1186/s12866-021-02226-x |
| PMID | 34090326 |
| PMC | PMC8180125 |
| MeSH | Animals Caenorhabditis elegans / drug effects* Caenorhabditis elegans / genetics Caenorhabditis elegans / immunology* Caenorhabditis elegans / microbiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / immunology Campylobacter Infections / drug therapy* Campylobacter Infections / genetics Campylobacter Infections / immunology Campylobacter Infections / microbiology Campylobacter jejuni / drug effects* Campylobacter jejuni / growth & development Chickens / genetics Chickens / immunology Chickens / microbiology Disease Models, Animal* Female Forkhead Transcription Factors / genetics Forkhead Transcription Factors / immunology Humans Lactobacillales / physiology* Mice / genetics Mice / immunology Mice / microbiology Mice, Inbred C57BL Nematoda / genetics Nematoda / immunology Nematoda / microbiology Probiotics / administration & dosage* |
| Resource | |
| General Microbes | JCM2009 JCM1101 JCM11657 JCM1132 |