| RRC ID |
71040
|
| Author |
Yoshida K, Hirano R, Sakai Y, Choi M, Sakanaka M, Kurihara S, Iino H, Xiao JZ, Katayama T, Odamaki T.
|
| Title |
Bifidobacterium response to lactulose ingestion in the gut relies on a solute-binding protein-dependent ABC transporter.
|
| Journal |
Commun Biol
|
| Abstract |
This study aims to understand the mechanistic basis underlying the response of Bifidobacterium to lactulose ingestion in guts of healthy Japanese subjects, with specific focus on a lactulose transporter. An in vitro assay using mutant strains of Bifidobacterium longum subsp. longum 105-A shows that a solute-binding protein with locus tag number BL105A_0502 (termed LT-SBP) is primarily involved in lactulose uptake. By quantifying faecal abundance of LT-SBP orthologues, which is defined by phylogenetic analysis, we find that subjects with 107 to 109 copies of the genes per gram of faeces before lactulose ingestion show a marked increase in Bifidobacterium after ingestion, suggesting the presence of thresholds between responders and non-responders to lactulose. These results help predict the prebiotics-responder and non-responder status and provide an insight into clinical interventions that test the efficacy of prebiotics.
|
| Volume |
4(1)
|
| Pages |
541
|
| Published |
2021-5-10
|
| DOI |
10.1038/s42003-021-02072-7
|
| PII |
10.1038/s42003-021-02072-7
|
| PMID |
33972677
|
| PMC |
PMC8110962
|
| MeSH |
ATP-Binding Cassette Transporters / metabolism*
Adolescent
Adult
Aged
Aged, 80 and over
Bacterial Proteins / metabolism*
Bifidobacterium / drug effects
Bifidobacterium / growth & development*
Bifidobacterium / metabolism
Cross-Sectional Studies
Feces / microbiology*
Female
Gastrointestinal Agents / administration & dosage
Gastrointestinal Microbiome / drug effects*
Gastrointestinal Tract / drug effects
Gastrointestinal Tract / microbiology*
Humans
Lactulose / administration & dosage*
Middle Aged
Young Adult
|
| Resource |
| General Microbes |
JCM1222
JCM12489
JCM1200
JCM1194
JCM1217
JCM1192
JCM15439
JCM7096
JCM1255
JCM8224 |
