| RRC ID |
71052
|
| Author |
Maejima Y, Iino T, Moriuchi R, Kushimoto K, Muraguchi Y, Fukuda K, Nojiri H, Ohkuma M, Dohra H, Kimbara K, Shintani M.
|
| Title |
Fluviispira sanaruensis sp., nov., Isolated from a Brackish Lake in Hamamatsu, Japan.
|
| Journal |
Curr Microbiol
|
| Abstract |
Strain RF1110005T, which was isolated from brackish lake water sampled at Lake Sanaru in Japan as a "filterable" bacterial strain, was characterized as a novel species in the genus Fluviispira, family Silvanigrellaceae, order Silvanigrellales, the class Oligoflexia and the phylum Bdellovibrionota. Cells of RF1110005T were aerobic, Gram stain negative, and show a pleomorphic morphology of spiral, filamentous and rod shapes. Catalase reaction was positive. Strain RF1110005T grew optimally at 30 °C, pH 7.0-8.0 and 0.5% NaCl (w/v). The major polar lipids in RF1110005T were phosphatidylethanolamine and phosphatidylglycerol. The predominant cellular fatty acids were iso-C15:0 and anteiso-C15:0. Phylogenetic analysis based on 16S rRNA gene sequences and concatenates of core gene sequence showed that the nearest neighbor of strain RF1110005T was Fluviispira multicolorata strain 33A1-SZDPT with 98.4% 16S rRNA gene sequence similarity. The genome size of strain RF1110005T was 3.5 Mbp with two plasmids (80 kb and 69 kb), and the G + C content was 33.7 mol%. Comparisons with genome-wide analyses and chemotaxonomic characters clearly showed that strain RF1110005T differed from F. multicolorata. Therefore, a novel species in Fluviispira sanaruensis, sp. nov., is proposed for strain RF1110005T (= JCM 31447 T = LMG 30360 T).
|
| Volume |
78(8)
|
| Pages |
3268-3276
|
| Published |
2021-8-1
|
| DOI |
10.1007/s00284-021-02561-2
|
| PII |
10.1007/s00284-021-02561-2
|
| PMID |
34086078
|
| MeSH |
Bacterial Typing Techniques
DNA, Bacterial / genetics
Fatty Acids / analysis
Genome-Wide Association Study*
Japan
Lakes*
Nucleic Acid Hybridization
Phospholipids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM31447
JCM32978 |
