論文 - 詳細
| RRC ID | 71064 |
|---|---|
| 著者 | Thin KK, He SW, Wang X, Wang Y, Rong M, Han JG, Zhang X. |
| タイトル | Rhizobium rhizolycopersici sp. nov., Isolated from the Rhizosphere Soil of Tomato Plants in China. |
| ジャーナル | Curr Microbiol |
| Abstract |
During characterization of rhizobacteria, strain DBTS2T was isolated from the rhizosphere soil samples of healthy tomato plants and characterized using a polyphasic taxonomic approach. Phylogenetic analysis using 16S rRNA gene sequences showed this strain belonged to the genus Rhizobium and was most closely related to Rhizobium subbaraonis JC85T (99.1%) and Rhizobium daejeonense CCBAU 10050T (97%). Cells of strain DBTS2T were Gram-negative, short rod, aerobic and non-motile. This novel strain was found to grow at 20-45 °C (optimum 25-37 °C), pH 5-9 (optimum 8) and in the presence of 4% NaCl. It was positive for catalase and oxidase. The predominant cellular fatty acids were Summed Feature 8 (52.7%) and C19:0 cyclo ω8c (23.3%). The polar lipids of strain DBTS2T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified aminophospholipid, unidentified aminolipid, four unidentified phospholipids, unidentified lipid, phosphatidylcholine, unknown glycolipid and unknown aminophosphoglycolipids. Q-10 was the major quinone. The DNA-DNA hybridization similarity values between the strain DBTS2T and R. subbaraonis JC85T, R. daejeonense CCBAU 10050T and Rhizobium azooxidifex DSM100211T were 46.4%, 20.7% and 25.5%, respectively. The ANI value was 91.96% between strain DBTS2T and R. subbaraonis JC85T and 75.18% between strain DBTS2T and R. daejeonense CCBAU 10050T. The DNA G+C content of the genomic DNA was 63.1 mol%. Based on these results, it was concluded that the isolate represents a novel species of the genus Rhizobium, for which the name Rhizobium rhizolycopersici sp. nov. is proposed, with DBTS2T (= CICC 24887T = ACCC61707 = JCM 34245) as the type strain. |
| 巻・号 | 78(2) |
| ページ | 830-836 |
| 公開日 | 2021-2-1 |
| DOI | 10.1007/s00284-020-02323-6 |
| PII | 10.1007/s00284-020-02323-6 |
| PMID | 33398443 |
| MeSH | Bacterial Typing Techniques China DNA, Bacterial / genetics Fatty Acids / analysis Phospholipids / analysis Phylogeny RNA, Ribosomal, 16S / genetics Rhizobium* / genetics Rhizosphere Sequence Analysis, DNA Soil Soil Microbiology Solanum lycopersicum* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM34245 |