論文 - 詳細
| RRC ID | 71075 |
|---|---|
| 著者 | Ungkulpasvich U, Baramee S, Uke A, Kosugi A. |
| タイトル | Symbiotic chitin degradation by a novel anaerobic thermophilic bacterium Hydrogenispora sp. UUS1-1 and the bacterium Tepidanaerobacter sp. GT38. |
| ジャーナル | Enzyme Microb Technol |
| Abstract |
Chitin is the second most abundant organic compound in nature. Although mesophilic bacteria degrade insoluble chitin, there is a paucity of data describing degradation of insoluble chitin by anaerobic thermophilic bacteria. In this report, we screened cow manure compost for new chitin degradation systems, and identified a chitinolytic bacterial community (CBC) that showed high chitin degradation activity under thermophilic conditions, i.e., 1% (w/v) chitin powder degraded completely within 7 days at 60 °C. Metagenomic analysis revealed that the CBC was dominated by two bacterial genera from Hydrogenispora, an uncultured taxonomic group, and Tepidanaerobacter. Hydrogenispora were abundant in the early-to-mid stages of culturing with chitin, whereas the population of Tepidanaerobacter increased during the later stages of culturing. Strains UUS1-1 and GT38, which were isolated as pure cultures using the roll-tube method with colloidal chitin, N-acetyl-d-glucosamine, and glucose as carbon sources, were found to be closely related to H. ethanolica and T. acetatoxydans, respectively. Strain UUS1-1 readily degraded chitin and is the first anaerobic thermophilic chitinolytic bacterium reported, whereas strain GT38 showed no chitinolytic activity. Based on phylogenetic analysis, UUS1-1 and GT38 should be classified as novel genera and species. Zymogram analysis revealed that UUS1-1 produces at least two chitinases with molecular weights of 150 and 40 kDa. A coculture of UUS1-1 and GT38 degraded crystalline chitin faster with lower accumulation of lactate compared with UUS1-1 alone, indicating that the strains maintained a symbiotic association through assimilation of organic acids in chitin degradation and that strain GT38 consumed end-products to reduce end-product inhibition and enhance the degradation of crystalline chitin. |
| 巻・号 | 144 |
| ページ | 109740 |
| 公開日 | 2021-3-1 |
| DOI | 10.1016/j.enzmictec.2020.109740 |
| PII | S0141-0229(20)30233-7 |
| PMID | 33541575 |
| MeSH | Anaerobiosis Bacteria / metabolism Chitin / metabolism Chitinases* / genetics Phylogeny |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM33882 JCM34212 |