Reference - Detail
| RRC ID | 71103 |
|---|---|
| Author | Lu P, Sui M, Zhang M, Wang M, Kamiya T, Okamoto K, Itoh H, Okuda S, Suzuki M, Asakura T, Fujiwara T, Nagata K. |
| Title | Rosmarinic Acid and Sodium Citrate Have a Synergistic Bacteriostatic Effect against Vibrio Species by Inhibiting Iron Uptake. |
| Journal | Int J Mol Sci |
| Abstract |
BACKGROUND:New strategies are needed to combat multidrug-resistant bacteria. The restriction of iron uptake by bacteria is a promising way to inhibit their growth. We aimed to suppress the growth of Vibrio bacterial species by inhibiting their ferric ion-binding protein (FbpA) using food components. METHODS:Twenty spices were selected for the screening of FbpA inhibitors. The candidate was applied to antibacterial tests, and the mechanism was further studied. RESULTS:An active compound, rosmarinic acid (RA), was screened out. RA binds competitively and more tightly than Fe3+ to VmFbpA, the FbpA from V. metschnikovii, with apparent KD values of 8 μM vs. 17 μM. Moreover, RA can inhibit the growth of V. metschnikovii to one-third of the control at 1000 μM. Interestingly, sodium citrate (SC) enhances the growth inhibition effect of RA, although SC only does not inhibit the growth. The combination of RA/SC completely inhibits the growth of not only V. metschnikovii at 100/100 μM but also the vibriosis-causative pathogens V. vulnificus and V. parahaemolyticus, at 100/100 and 1000/100 μM, respectively. However, RA/SC does not affect the growth of Escherichia coli. CONCLUSIONS:RA/SC is a potential bacteriostatic agent against Vibrio species while causing little damage to indigenous gastrointestinal bacteria. |
| Volume | 22(23) |
| Published | 2021-12-1 |
| DOI | 10.3390/ijms222313010 |
| PII | ijms222313010 |
| PMID | 34884815 |
| PMC | PMC8657459 |
| MeSH | Bacterial Proteins / chemistry Bacterial Proteins / metabolism Binding Sites Cinnamates / chemistry Cinnamates / metabolism Cinnamates / pharmacology* Depsides / chemistry Depsides / metabolism Depsides / pharmacology* Drug Synergism Iron / metabolism* Iron-Binding Proteins / chemistry Iron-Binding Proteins / metabolism Molecular Docking Simulation Plant Extracts / chemistry Protein Binding Rosmarinic Acid Sodium Citrate / pharmacology* Vibrio parahaemolyticus / drug effects* Vibrio parahaemolyticus / metabolism |
| Resource | |
| General Microbes | JCM21189 JCM3725 JCM32818 |