| RRC ID |
71285
|
| Author |
Tsukuda N, Yahagi K, Hara T, Watanabe Y, Matsumoto H, Mori H, Higashi K, Tsuji H, Matsumoto S, Kurokawa K, Matsuki T.
|
| Title |
Key bacterial taxa and metabolic pathways affecting gut short-chain fatty acid profiles in early life.
|
| Journal |
ISME J
|
| Abstract |
Infant gut microbiota development affects the host physiology throughout life, and short-chain fatty acids (SCFAs) are promising key metabolites mediating microbiota-host relationships. Here, we investigated dense longitudinally collected faecal samples from 12 subjects during the first 2 years (n = 1048) to identify early life gut SCFA patterns and their relationships with the microbiota. Our results revealed three distinct phases of progression in the SCFA profiles: early phase characterised by low acetate and high succinate, middle-phase characterised by high lactate and formate and late-phase characterised by high propionate and butyrate. Assessment of the SCFA-microbiota relationships revealed that faecal butyrate is associated with increased Clostridiales and breastfeeding cessation, and that diverse and personalised assemblage of Clostridiales species possessing the acetyl-CoA pathway play major roles in gut butyrate production. We also found an association between gut formate and some infant-type bifidobacterial species, and that human milk oligosaccharides (HMO)-derived fucose is the substrate for formate production during breastfeeding. We identified genes upregulated in fucose and fucosylated HMO utilisation in infant-type bifidobacteria. Notably, bifidobacteria showed interspecific and intraspecific variation in the gene repertoires, and cross-feeding of fucose contributed to gut formate production. This study provides an insight into early life SCFA-microbiota relationships, which is an important step for developing strategies for modulating lifelong health.
|
| Volume |
15(9)
|
| Pages |
2574-2590
|
| Published |
2021-9-1
|
| DOI |
10.1038/s41396-021-00937-7
|
| PII |
10.1038/s41396-021-00937-7
|
| PMID |
33723382
|
| PMC |
PMC8397723
|
| MeSH |
Clostridiales
Fatty Acids, Volatile*
Female
Gastrointestinal Microbiome*
Humans
Infant
Metabolic Networks and Pathways
Milk, Human
|
| Resource |
| General Microbes |
JCM1192
JCM1222
JCM1217
JCM1194
JCM15439 |
