| RRC ID |
71315
|
| Author |
Katsuki R, Shiraishi T, Sakata S, Hirota T, Nakamura Y, Yokota SI.
|
| Title |
Inhibitory Effect of the Glycerophosphate Moiety of Lipoteichoic Acid from Lactic Acid Bacteria on Dexamethasone-Induced Atrogin-1 Expression in C2C12 Myotubes.
|
| Journal |
J Nutr Sci Vitaminol (Tokyo)
|
| Abstract |
Atrogin-1, which is an important regulator of ubiquitin-mediated protein degradation in skeletal muscle, is a major marker of muscle loss and disuse muscle atrophy. To investigate which components of lactic acid bacteria (LAB) suppress dexamethasone (DEX)-induced atrogin-1 expression, mouse skeletal muscle C2C12 myotubes were treated with DEX in the presence or absence of components of LAB. Heat-killed cells and lipoteichoic acid (LTA) derived from five LAB strains significantly suppressed DEX-induced atrogin-1 expression. The glycerophosphate (GroP) fraction prepared from chemically-degraded LTA and sn-glycerol-1-phosphate suppressed DEX-induced atrogin-1 expression, whereas the glycolipid anchor fraction of LTA did not. Heat-killed cells obtained by culturing under low-Mn2+ conditions, which generated fewer poly-GroP polymers in LTA, displayed significantly lower inhibitory activity compared to heat-killed cells grown under normal conditions. These results suggested that LTA of LAB contributed to suppressing atrogin-1 expression and that the GroP moiety of LTA was responsible for its inhibitory activity.
|
| Volume |
67(5)
|
| Pages |
351-357
|
| Published |
2021-1-1
|
| DOI |
10.3177/jnsv.67.351
|
| PMID |
34719621
|
| MeSH |
Animals
Dexamethasone / pharmacology
Glycerophosphates
Lactobacillales*
Lipopolysaccharides
Mice
Muscle Fibers, Skeletal
Muscle Proteins
Muscle, Skeletal / pathology
Muscular Atrophy* / pathology
SKP Cullin F-Box Protein Ligases
Teichoic Acids
Ubiquitin-Protein Ligases
|
| Resource |
| General Microbes |
JCM1131
JCM1136
JCM1149
JCM1558
JCM1559 |
