Reference - Detail
| RRC ID | 71316 |
|---|---|
| Author | Fukuoka T, Nakamura S, Morita T, Ohmura T, Kotani M, Naito Y, Sato H. |
| Title | Surface-assisted Laser Desorption/ionization Mass Spectrometry Analysis of the Glycolipid Biosurfactants, Mannosylerythritol Lipids, Using an Ionization-assisting Substrate. |
| Journal | J Oleo Sci |
| Abstract |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for the screening of glycolipid-type biosurfactants (BSs) from a crude extract of microbial products. However, it is unsuitable for the detection of lower molecular weight products because the observed ions are overlapped with matrix-derived ions at lower mass range. In this study, we applied a "matrix-free" surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) analysis using a through-hole alumina membrane as an ionization-assisting substrate. Using this method, we could detect a variety of lower molecular weight products in an extract of a glycolipid BS producer with good sensitivity. In addition, the culture solution could be analyzed directly by this method. |
| Volume | 70(8) |
| Pages | 1175-1179 |
| Published | 2021-8-5 |
| DOI | 10.5650/jos.ess21084 |
| PMID | 34248100 |
| MeSH | Aluminum Oxide / chemistry Basidiomycota / metabolism Glycolipids / analysis* Glycolipids / biosynthesis Glycolipids / chemistry Mass Spectrometry / instrumentation Mass Spectrometry / methods Membranes, Artificial Molecular Weight Surface-Active Agents / analysis* Surface-Active Agents / chemistry Surface-Active Agents / metabolism |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM10317 |