| RRC ID |
71316
|
| Author |
Fukuoka T, Nakamura S, Morita T, Ohmura T, Kotani M, Naito Y, Sato H.
|
| Title |
Surface-assisted Laser Desorption/ionization Mass Spectrometry Analysis of the Glycolipid Biosurfactants, Mannosylerythritol Lipids, Using an Ionization-assisting Substrate.
|
| Journal |
J Oleo Sci
|
| Abstract |
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for the screening of glycolipid-type biosurfactants (BSs) from a crude extract of microbial products. However, it is unsuitable for the detection of lower molecular weight products because the observed ions are overlapped with matrix-derived ions at lower mass range. In this study, we applied a "matrix-free" surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) analysis using a through-hole alumina membrane as an ionization-assisting substrate. Using this method, we could detect a variety of lower molecular weight products in an extract of a glycolipid BS producer with good sensitivity. In addition, the culture solution could be analyzed directly by this method.
|
| Volume |
70(8)
|
| Pages |
1175-1179
|
| Published |
2021-8-5
|
| DOI |
10.5650/jos.ess21084
|
| PMID |
34248100
|
| MeSH |
Aluminum Oxide / chemistry
Basidiomycota / metabolism
Glycolipids / analysis*
Glycolipids / biosynthesis
Glycolipids / chemistry
Mass Spectrometry / instrumentation
Mass Spectrometry / methods
Membranes, Artificial
Molecular Weight
Surface-Active Agents / analysis*
Surface-Active Agents / chemistry
Surface-Active Agents / metabolism
|
| Resource |
| General Microbes |
JCM10317 |
