RRC ID 7137
Author Iwamori T, Nukumi N, Itoh K, Kano K, Naito K, Kurohmaru M, Yamanouchi K, Tojo H.
Title Bacteriostatic activity of Whey Acidic Protein (WAP).
Journal J. Vet. Med. Sci.
Abstract We have previously reported the action of whey acidic protein (WAP) inhibiting the proliferation of mouse mammary epithelial cells in the experiments utilizing in vivo and in vitro systems. We report herein the bacteriostatic activity of WAP. Western blot analysis demonstrated successful isolation of WAP from whey fractions of rat milk by column chromatography. The WAP fraction inhibited the growth of Staphylococcus aureus JCM2413 in a dose-dependent manner, but did not inhibit the growth of Escherichia coli. The bacteriostatic activity of WAP was highest at pH 6.6 and was not affected by the presence of 150 mM NaCl. A scanning electron micrograph of bacteria treated with WAP exhibited the disruption of the bacterial cell walls.
Volume 72(5)
Pages 621-5
Published 2010-5
PII JST.JSTAGE/jvms/08-0331
PMID 20009425
MeSH Animals Anti-Bacterial Agents / pharmacology* Cell Division / drug effects Epithelial Cells / cytology Epithelial Cells / drug effects Mammary Glands, Animal / cytology Mammary Glands, Animal / drug effects Mice Microscopy, Electron, Scanning Milk / chemistry Milk Proteins / isolation & purification Milk Proteins / pharmacology* Rats Recombinant Proteins / pharmacology Staphylococcus aureus / drug effects Staphylococcus aureus / ultrastructure
IF 0.803
Times Cited 7
General Microbes JCM 2413 JCM 5491