| RRC ID |
71383
|
| 著者 |
Udayan S, Buttó LF, Rossini V, Velmurugan J, Martinez-Lopez M, Sancho D, Melgar S, O'Toole PW, Nally K.
|
| タイトル |
Macrophage cytokine responses to commensal Gram-positive Lactobacillus salivarius strains are TLR2-independent and Myd88-dependent.
|
| ジャーナル |
Sci Rep
|
| Abstract |
The mechanisms through which cells of the host innate immune system distinguish commensal bacteria from pathogens are currently unclear. Toll-like receptors (TLRs) are a class of pattern recognition receptors (PRRs) expressed by host cells which recognize microbe-associated molecular patterns (MAMPs) common to both commensal and pathogenic bacteria. Of the different TLRs, TLR2/6 recognize bacterial lipopeptides and trigger cytokines responses, especially to Gram-positive and Gram-negative pathogens. We report here that TLR2 is dispensable for triggering macrophage cytokine responses to different strains of the Gram-positive commensal bacterial species Lactobacillus salivarius. The L. salivarius UCC118 strain strongly upregulated expression of the PRRs, Mincle (Clec4e), TLR1 and TLR2 in macrophages while downregulating other TLR pathways. Cytokine responses triggered by L. salivarius UCC118 were predominantly TLR2-independent but MyD88-dependent. However, macrophage cytokine responses triggered by another Gram-positive commensal bacteria, Bifidobacterium breve UCC2003 were predominantly TLR2-dependent. Thus, we report a differential requirement for TLR2-dependency in triggering macrophage cytokine responses to different commensal Gram-positive bacteria. Furthermore, TNF-α responses to the TLR2 ligand FSL-1 and L. salivarius UCC118 were partially Mincle-dependent suggesting that PRR pathways such as Mincle contribute to the recognition of MAMPs on distinct Gram-positive commensal bacteria. Ultimately, integration of signals from these different PRR pathways and other MyD88-dependent pathways may determine immune responses to commensal bacteria at the host-microbe interface.
|
| 巻・号 |
11(1)
|
| ページ |
5896
|
| 公開日 |
2021-3-15
|
| DOI |
10.1038/s41598-021-85347-7
|
| PII |
10.1038/s41598-021-85347-7
|
| PMID |
33723368
|
| PMC |
PMC7961041
|
| MeSH |
Animals
Cytokines / metabolism*
Humans
Ligands
Ligilactobacillus salivarius / physiology*
Macrophages / metabolism*
Macrophages / microbiology*
Male
Mice
Mice, Inbred C57BL
Myeloid Differentiation Factor 88 / metabolism*
NF-kappa B / metabolism
Receptors, Pattern Recognition / metabolism
THP-1 Cells
Toll-Like Receptor 2 / agonists
Toll-Like Receptor 2 / metabolism*
|
| リソース情報 |
| 一般微生物 |
JCM1040
JCM1042
JCM1044
JCM1045
JCM1046
JCM1047
JCM1230 |
