Reference - Detail
| RRC ID | 7139 |
|---|---|
| Author | Nadkarni MA, Simonian MR, Harty DW, Zoellner H, Jacques NA, Hunter N. |
| Title | Lactobacilli are prominent in the initial stages of polymicrobial infection of dental pulp. |
| Journal | J Clin Microbiol |
| Abstract |
In earlier studies we used molecular methods to identify the major bacterial consortia associated with advanced dentin caries. These consortia are dominated by bacteria from the families Lactobacillaceae, Streptococcaceae, Veillonellaceae (formerly Acidaminococcaceae), Eubacteriaceae, and Lachnospiraceae from the phylum Firmicutes; Coriobacteriaceae, Bifidobacteriaceae, and Propionibacteriaceae from the phylum Actinobacteria; and Prevotellaceae from the phylum Bacteroidetes, as well as fusobacteria. The phases of infection of vital pulp tissue by dentin microorganisms remain obscure. In the present study, fluorescence in situ hybridization was performed on sections of tissue embedded in resin. Probes for 16S rRNA corresponding to the major taxa of bacteria in carious dentin were used to provide information on the characteristics of pulp infection. Lactobacilli were prominent in 7 of 8 pulps determined to be at a limited stage of infection. Established infection (6 pulps) showed a more complex profile, with lactobacilli persisting in all of the lesions and with invasion of the necrotic regions of tissue by Bacteroidetes, fusobacteria, Lachnospiraceae, and Coriobacteriaceae in particular. Advanced infections (7 pulps) were characterized by mixed anaerobic species, with a strong representation by Coriobacteriaceae and Lachnospiraceae. Lactobacilli were not represented at this stage. Typically, groups of organisms were spatially isolated within the pulp tissue. Analysis indicated that lactobacilli could invade vital pulp tissue to achieve a very high biomass that was not associated with a detectable local inflammatory infiltrate. The findings establish that invasion of the dental pulp can be associated with a pronounced selection from the complex microbial populations within carious dentin, suggesting specific pathogenicity. |
| Volume | 48(5) |
| Pages | 1732-40 |
| Published | 2010-5-1 |
| DOI | 10.1128/JCM.01912-09 |
| PII | JCM.01912-09 |
| PMID | 20200294 |
| PMC | PMC2863874 |
| MeSH | Adult Bacteria / genetics Bacteria / isolation & purification* Biodiversity* Dental Pulp / microbiology* Female Humans In Situ Hybridization, Fluorescence Male Oligonucleotide Probes / genetics Pulpitis / microbiology* RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics |
| IF | 5.897 |
| Times Cited | 15 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 6480 JCM 6425 JCM 8545 JCM 14553 JCM 10299 JCM 10300 JCM 6330 JCM 6290 JCM 12541 JCM 12084 JCM 12083 JCM 12245 |