RRC ID |
7144
|
Author |
Hotta Y, Teramoto K, Sato H, Yoshikawa H, Hosoda A, Tamura H.
|
Title |
Classification of genus Pseudomonas by MALDI-TOF MS based on ribosomal protein coding in S10-spc-alpha operon at strain level.
|
Journal |
J Proteome Res
|
Abstract |
We have proposed a rapid phylogenetic classification at the strain level by MALDI-TOF MS using ribosomal protein matching profiling. In this study, the S10-spc-alpha operon, encoding half of the ribosomal subunit proteins and highly conserved in eubacterial genomes, was selected for construction of the ribosomal protein database as biomarkers for bacterial identification by MALDI-TOF MS analysis to establish a more reliable phylogenetic classification. Our method revealed that the 14 reliable and reproducible ribosomal subunit proteins with less than m/z 15,000, except for L14, coded in the S10-spc-alpha operon were significantly useful biomarkers for bacterial classification at species and strain levels by MALDI-TOF MS analysis of genus Pseudomonas strains. The obtained phylogenetic tree was consisted with that based on genetic sequence (gyrB). Since S10-spc-alpha operons of genus Pseudomonas strains were sequenced using specific primers designed based on nucleotide sequences of genome-sequenced strains, the ribosomal subunit proteins encoded in S10-spc-alpha operon were suitable biomarkers for construction and correction of the database. MALDI-TOF MS analysis using these 14 selected ribosomal proteins is a rapid, efficient, and versatile bacterial identification method with the validation procedure for the obtained results.
|
Volume |
9(12)
|
Pages |
6722-8
|
Published |
2010-12-3
|
DOI |
10.1021/pr100868d
|
PMID |
20945934
|
MeSH |
Bacterial Proteins / analysis*
Bacterial Proteins / genetics
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Databases, Protein
Molecular Sequence Data
Operon
Phylogeny
Protein Subunits / analysis
Protein Subunits / genetics
Pseudomonas / classification
Pseudomonas / genetics
Pseudomonas / metabolism*
Ribosomal Proteins / analysis*
Ribosomal Proteins / genetics
Sequence Analysis, DNA
Species Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
|
IF |
4.074
|
Times Cited |
29
|
WOS Category
|
BIOCHEMICAL RESEARCH METHODS
|
Resource |
General Microbes |
JCM 6156
JCM 13061 |