RRC ID 71502
Author Jiménez-Guerrero I, Acosta-Jurado S, Medina C, Ollero FJ, Alias-Villegas C, Vinardell JM, Pérez-Montaño F, López-Baena FJ.
Title The Sinorhizobium fredii HH103 type III secretion system effector NopC blocks nodulation with Lotus japonicus Gifu.
Journal J Exp Bot
Abstract The broad-host-range bacterium Sinorhizobium fredii HH103 cannot nodulate the model legume Lotus japonicus Gifu. This bacterium possesses a type III secretion system (T3SS), a specialized secretion apparatus used to deliver effector proteins (T3Es) into the host cell cytosol to alter host signaling and/or suppress host defence responses to promote infection. However, some of these T3Es are recognized by specific plant receptors and hence trigger a strong defence response to block infection. In rhizobia, T3Es are involved in nodulation efficiency and host-range determination, and in some cases directly activate host symbiosis signalling in a Nod factor-independent manner. In this work, we show that HH103 RifR T3SS mutants, unable to secrete T3Es, gain nodulation with L. japonicus Gifu through infection threads, suggesting that plant recognition of a T3E could block the infection process. To identify the T3E involved, we performed nodulation assays with a collection of mutants that affect secretion of each T3E identified in HH103 RifR so far. The nopC mutant could infect L. japonicus Gifu by infection thread invasion and switch the infection mechanism in Lotus burttii from intercellular infection to infection thread formation. Lotus japonicus gene expression analysis indicated that the infection-blocking event occurs at early stages of the symbiosis.
Volume 71(19)
Pages 6043-6056
Published 2020-10-7
DOI 10.1093/jxb/eraa297
PII 5863328
PMID 32589709
MeSH Bacterial Proteins / genetics Lotus* Plant Root Nodulation Sinorhizobium* Sinorhizobium fredii* / genetics Symbiosis Type III Secretion Systems
IF 5.908
Lotus / Glycine Gifu B-129