RRC ID 71545
Author Sun J, Li X, Qiu Y, Xue X, Zhang M, Yang W, Zhou D, Hu L, Lu R, Zhang Y.
Title Quorum sensing regulates transcription of the pilin gene mshA1 of MSHA pilus in Vibrio parahaemolyticus.
Journal Gene
Abstract Vibrio parahaemolyticus produces two types of IV pili: mannose-sensitive haemagglutinin type IV pili (MSHA) and chitin-regulated pili (ChiRP). Both of them are required for biofilm formation and the pathogen persistence in hosts. However, there are few reports on the regulation of their expression. In the present study, we showed that the master quorum sensing (QS) regulators AphA and OpaR oppositely regulated the transcription of mshA1 encoding the pilin of MSHA pilus in V. parahaemolyticus. At low cell density (LCD), AphA indirectly repressed mshA1 transcription. In contrast, at high cell density (HCD), OpaR bound to the regulatory DNA region of mshA1 to activate its transcription. Oppositely regulation of mshA1 by AphA and OpaR led to a gradual increase in the expression level of mshA1 from LCD to HCD. Thus, regulation of type IV pili production was one of the mechanisms that V. parahaemolyticus adopted to control biofilm formation.
Volume 807
Pages 145961
Published 2022-1-10
DOI 10.1016/j.gene.2021.145961
PII S0378-1119(21)00556-4
PMID 34530088
MeSH Bacterial Proteins / genetics Biofilms / growth & development Cell Count Fimbriae Proteins / genetics* Fimbriae Proteins / metabolism Gene Expression / genetics Gene Expression Regulation, Bacterial / genetics Mannose-Binding Lectin / genetics Mannose-Binding Lectin / metabolism Quorum Sensing / genetics* Trans-Activators / genetics Trans-Activators / metabolism Transcription Factors / genetics Transcription Factors / metabolism Vibrio / genetics* Vibrio / metabolism Vibrio cholerae / genetics Vibrio cholerae / metabolism Vibrio parahaemolyticus / genetics Vibrio parahaemolyticus / metabolism
IF 2.984
Resource
Pathogenic bacteria V. parahaemolyticus RIMD 2210633