RRC ID 71548
Author Prithvisagar KS, Krishna Kumar B, Kodama T, Rai P, Iida T, Karunasagar I, Karunasagar I.
Title Whole genome analysis unveils genetic diversity and potential virulence determinants in Vibrio parahaemolyticus associated with disease outbreak among cultured Litopenaeus vannamei (Pacific white shrimp) in India.
Journal Virulence
Abstract Vibrio parahaemolyticus has caused widespread mortality in Indian shrimp aquaculture in recent years. However, there are insufficient genome data for the isolates from Indian shrimp vibriosis to analyze genetic diversity and track the acquisition of genetic features that could be involved in virulence and fitness. In this study, we have performed genome analysis of V. parahaemolyticus isolated from moribund shrimps collected from shrimp farms along coastal Karnataka, India, for better understanding of their diversity and virulence. Five newly sequenced genomes of V. parahaemolyticus along with 40 genomes retrieved from NCBI were subjected to comparative genome analysis. The sequenced genomes had an overall genome size of 5.2 Mb. MLST analysis and core genome phylogenomic analysis revealed considerable genetic diversity among the isolates obtained from the moribund shrimps. Interestingly, none of the V. parahaemolyticus isolates possessed the classical features (PirAB) of the strains associated with Acute Hepatopancreatic Necrosis Disease (AHPND). This study also revealed the presence of multiple virulence attributes, including ZOT, ACE and RTX toxins, secretion systems, and mobile genetic elements. The findings of this study provide insights into the possible transition of an environmental V. parahaemolyticus to emerge as pathogens of aquaculture species by increasing its virulence and host adaptation. Future studies focusing on continuous genomic surveillance of V. parahaemolyticus are required to study the evolution and transmission of new variants in shrimp aquaculture, as well as to design and implement biosecurity programs to prevent disease outbreaks.
Volume 12(1)
Pages 1936-1949
Published 2021-12-1
DOI 10.1080/21505594.2021.1947448
PMID 34415829
PMC PMC8381830
MeSH Animals Aquaculture Disease Outbreaks / veterinary Genetic Variation India / epidemiology Multilocus Sequence Typing Penaeidae* / microbiology Vibrio parahaemolyticus* / genetics Vibrio parahaemolyticus* / pathogenicity Virulence* / genetics Whole Genome Sequencing
IF 5.542
Pathogenic bacteria V. parahaemolyticus RIMD2210633