論文 - 詳細
| RRC ID | 71567 |
|---|---|
| 著者 | Satoda Y, Noguchi T, Fujii T, Taniguchi A, Katoh Y, Nakayama K. |
| タイトル | BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36 contributes to intraflagellar transport turnaround involving ICK/CILK1. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Primary cilia are antenna-like organelles that contain specific proteins, and are crucial for tissue morphogenesis. Anterograde and retrograde trafficking of ciliary proteins are mediated by the intraflagellar transport (IFT) machinery. BROMI/TBC1D32 interacts with CCRK/CDK20, which phosphorylates and activates the ICK/CILK1 kinase, to regulate the change in direction of the IFT machinery at the ciliary tip. Mutations in BROMI, CCRK, and ICK in humans cause ciliopathies, and mice defective in these genes are also known to demonstrate ciliopathy phenotypes. We here show that BROMI interacts not only with CCRK but also with CFAP20, an evolutionarily conserved ciliary protein, and with FAM149B1/JBTS36, a protein in which mutations cause Joubert syndrome. In addition, we show that FAM149B1 interacts directly with CCRK as well as with BROMI. Ciliary defects observed in CCRK-knockout (KO), BROMI-KO, and FAM149B1-KO cells, including abnormally long cilia and accumulation of the IFT machinery and ICK at the ciliary tip, resembled one another, and BROMI mutants that are defective in binding to CCRK and CFAP20 were unable to rescue the ciliary defects of BROMI-KO cells. These data indicate that CCRK, BROMI, FAM149B1, and probably CFAP20, all together regulate the IFT turnaround process under the control of ICK. [Media: see text] [Media: see text]. |
| 巻・号 | 33(9) |
| ページ | ar79 |
| 公開日 | 2022-8-1 |
| DOI | 10.1091/mbc.E22-03-0089 |
| PMID | 35609210 |
| PMC | PMC9582636 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism Animals Biological Transport Cilia / metabolism Ciliopathies* / metabolism Cyclin-Dependent Kinase-Activating Kinase Cyclin-Dependent Kinases Cytoskeletal Proteins Eye Abnormalities* / metabolism Humans Kidney Diseases, Cystic* / metabolism Mice Protein Serine-Threonine Kinases Protein Transport Proteins / metabolism |
| IF | 3.791 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |