RRC ID 71598
Author Aoki H, Ohnishi K, Wang X, Takahashi A, Ohnishi T, Nakamura M, Sakaki T.
Title p53-independent WAF1 induction by ACNU in human glioblastoma cells.
Journal Mol Carcinog
Abstract The induction of WAF1 gene expression after the treatment with the anticancer agent 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU; nimustine hydrochloride) was studied in two human glioblastoma cell lines: U-87MG, which bears the wild-type p53 gene, and T98G, which bears the mutant p53 gene. A marked accumulation of WAF1 was observed 3 h after ACNU treatment in both cell lines. The induction of WAF1 mRNA by ACNU was detected by northern blot analysis in these cells. Binding activity of p53 to a p53 consensus sequence increased after treatment in U-87MG cells but not in T98G cells. The existence of a p53-independent WAF1 induction pathway was supported by the apparent accumulation of WAF1 after ACNU treatment in the p53-null human osteosarcoma cell line Saos-2. These findings suggest that there are two possible pathways for WAF1 induction: the p53-dependent pathway through the p53-responsive element and the p53-independent pathway through other elements.
Volume 21(3)
Pages 171-6
Published 1998-3-1
DOI 10.1002/(sici)1098-2744(199803)21:3<171::aid-mc4>3.0.co;2-p
PII 10.1002/(SICI)1098-2744(199803)21:3<171::AID-MC4>3.0.CO;2-P
PMID 9537648
MeSH Antineoplastic Agents / pharmacology* Blotting, Northern Blotting, Western Cyclin-Dependent Kinase Inhibitor p21 Cyclins / biosynthesis* Gene Expression Regulation, Neoplastic / drug effects Genes, p53 Glioblastoma / drug therapy* Glioblastoma / metabolism* Humans Nimustine / pharmacology* Tumor Cells, Cultured Tumor Suppressor Protein p53 / biosynthesis Tumor Suppressor Protein p53 / physiology*
IF 3.825
Human and Animal Cells U-87 MG(RCB0419)