| RRC ID |
71598
|
| 著者 |
Aoki H, Ohnishi K, Wang X, Takahashi A, Ohnishi T, Nakamura M, Sakaki T.
|
| タイトル |
p53-independent WAF1 induction by ACNU in human glioblastoma cells.
|
| ジャーナル |
Mol Carcinog
|
| Abstract |
The induction of WAF1 gene expression after the treatment with the anticancer agent 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU; nimustine hydrochloride) was studied in two human glioblastoma cell lines: U-87MG, which bears the wild-type p53 gene, and T98G, which bears the mutant p53 gene. A marked accumulation of WAF1 was observed 3 h after ACNU treatment in both cell lines. The induction of WAF1 mRNA by ACNU was detected by northern blot analysis in these cells. Binding activity of p53 to a p53 consensus sequence increased after treatment in U-87MG cells but not in T98G cells. The existence of a p53-independent WAF1 induction pathway was supported by the apparent accumulation of WAF1 after ACNU treatment in the p53-null human osteosarcoma cell line Saos-2. These findings suggest that there are two possible pathways for WAF1 induction: the p53-dependent pathway through the p53-responsive element and the p53-independent pathway through other elements.
|
| 巻・号 |
21(3)
|
| ページ |
171-6
|
| 公開日 |
1998-3-1
|
| DOI |
10.1002/(sici)1098-2744(199803)21:3<171::aid-mc4>3.0.co;2-p
|
| PII |
10.1002/(SICI)1098-2744(199803)21:3<171::AID-MC4>3.0.CO;2-P
|
| PMID |
9537648
|
| MeSH |
Antineoplastic Agents / pharmacology*
Blotting, Northern
Blotting, Western
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / biosynthesis*
Gene Expression Regulation, Neoplastic / drug effects
Genes, p53
Glioblastoma / drug therapy*
Glioblastoma / metabolism*
Humans
Nimustine / pharmacology*
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / biosynthesis
Tumor Suppressor Protein p53 / physiology*
|
| IF |
3.825
|
| リソース情報 |
| ヒト・動物細胞 |
U-87 MG(RCB0419) |
