Reference - Detail
| RRC ID | 71613 |
|---|---|
| Author | Tashiro M, Yoshida Y, Oike T, Nakao M, Yusa K, Hirota Y, Ohno T. |
| Title | First Human Cell Experiments With FLASH Carbon Ions. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND/AIM:This study aimed to establish a setup for ultra-high-dose-rate (FLASH) carbon-ion irradiation, and to conduct the first human cell experiments using FLASH carbon ions. MATERIALS AND METHODS:A system for FLASH carbon-ion irradiation (1-3 Gy at 13 or 50 keV/μm) was developed. The growth and senescence of HFL1 lung fibroblasts were assessed by crystal violet staining assays and senescence-associated β-galactosidase staining, respectively. Survival of HSGc-C5 cancer cells was assessed by clonogenic assays. RESULTS:The dose rates of carbon ions ranged from 96-195 Gy/s, meeting the definition of FLASH. With both 13 and 50 keV/μm beams, no FLASH sparing effect was observed on the growth suppression and senescence of HFL1 cells, nor on the survival of HSGc-C5 cells. CONCLUSION:We successfully conducted the first human cell experiments with FLASH carbon ions. No FLASH effect was observed under the conditions examined. |
| Volume | 42(5) |
| Pages | 2469-2477 |
| Published | 2022-5-1 |
| DOI | 10.21873/anticanres.15725 |
| PII | 42/5/2469 |
| PMID | 35489744 |
| MeSH | Carbon* Fibroblasts / radiation effects Heavy Ion Radiotherapy* Humans Ions |
| IF | 1.994 |
| Resource | |
| Human and Animal Cells | HFL-I(RCB0521) |