論文 - 詳細
| RRC ID | 71633 |
|---|---|
| 著者 | Fukusaki E, Oishi T, Tanaka H, Kajiyama S, Kobayashi A. |
| タイトル | Identification of genes induced by taxol application using a combination of differential display RT-PCR and DNA microarray analysis. |
| ジャーナル | Z Naturforsch C J Biosci |
| Abstract |
The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis. |
| 巻・号 | 56(9-10) |
| ページ | 814-9 |
| 公開日 | 2001-1-1 |
| DOI | 10.1515/znc-2001-9-1022 |
| PMID | 11724388 |
| MeSH | Cloning, Molecular Gene Expression Profiling / methods* Gene Expression Regulation* / drug effects* HeLa Cells / drug effects Humans Oligonucleotide Array Sequence Analysis* Paclitaxel / pharmacology* Reverse Transcriptase Polymerase Chain Reaction* Sequence Homology, Nucleic Acid |
| IF | 1.238 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |