論文 - 詳細
| RRC ID | 71653 |
|---|---|
| 著者 | Mori N, Oishi K, Sar B, Mukaida N, Nagatake T, Matsushima K, Yamamoto N. |
| タイトル | Essential role of transcription factor nuclear factor-kappaB in regulation of interleukin-8 gene expression by nitrite reductase from Pseudomonas aeruginosa in respiratory epithelial cells. |
| ジャーナル | Infect Immun |
| Abstract |
Persistent infection with Pseudomonas aeruginosa increases interleukin-8 (IL-8) levels and causes dense neutrophil infiltrations in the airways of patients with chronic airway diseases. Recently, we have reported that nitrite reductase from P. aeruginosa induces the production of IL-8 in respiratory cells, including bronchial epithelial cells. To determine the molecular mechanism(s) of nitrite reductase-induced IL-8 expression in respiratory cells, A549 epithelial cells were transfected with plasmids containing serial deletions of the 5'-flanking region of the IL-8 gene and then exposed to nitrite reductase. Nitrite reductase significantly enhanced IL-8 gene promoter-driven reporter activity. This increased IL-8 gene expression was inhibited by mutating the nuclear factor-kappaB (NF-kappaB) binding element. Nitrite reductase enhanced nuclear localization of the NF-kappaB binding complex. Furthermore, nitrite reductase induced the degradation of IkappaBalpha, the major cytoplasmic inhibitor of NF-kappaB, and the expression of IkappaBalpha mRNA. These data support the critical role of the activation of NF-kappaB in nitrite reductase-induced IL-8 gene expression in airway epithelium. |
| 巻・号 | 67(8) |
| ページ | 3872-8 |
| 公開日 | 1999-8-1 |
| DOI | 10.1128/IAI.67.8.3872-3878.1999 |
| PMID | 10417151 |
| PMC | PMC96667 |
| MeSH | DNA-Binding Proteins / metabolism Epithelial Cells / metabolism Gene Expression Regulation / drug effects* Humans I-kappa B Proteins* Interleukin-8 / genetics* Lung / metabolism* NF-KappaB Inhibitor alpha NF-kappa B / physiology* Nitrite Reductases / pharmacology* Promoter Regions, Genetic Pseudomonas aeruginosa / enzymology* Transcription Factor AP-1 / physiology Tumor Cells, Cultured |
| IF | 3.201 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |