| RRC ID |
71653
|
| 著者 |
Mori N, Oishi K, Sar B, Mukaida N, Nagatake T, Matsushima K, Yamamoto N.
|
| タイトル |
Essential role of transcription factor nuclear factor-kappaB in regulation of interleukin-8 gene expression by nitrite reductase from Pseudomonas aeruginosa in respiratory epithelial cells.
|
| ジャーナル |
Infect Immun
|
| Abstract |
Persistent infection with Pseudomonas aeruginosa increases interleukin-8 (IL-8) levels and causes dense neutrophil infiltrations in the airways of patients with chronic airway diseases. Recently, we have reported that nitrite reductase from P. aeruginosa induces the production of IL-8 in respiratory cells, including bronchial epithelial cells. To determine the molecular mechanism(s) of nitrite reductase-induced IL-8 expression in respiratory cells, A549 epithelial cells were transfected with plasmids containing serial deletions of the 5'-flanking region of the IL-8 gene and then exposed to nitrite reductase. Nitrite reductase significantly enhanced IL-8 gene promoter-driven reporter activity. This increased IL-8 gene expression was inhibited by mutating the nuclear factor-kappaB (NF-kappaB) binding element. Nitrite reductase enhanced nuclear localization of the NF-kappaB binding complex. Furthermore, nitrite reductase induced the degradation of IkappaBalpha, the major cytoplasmic inhibitor of NF-kappaB, and the expression of IkappaBalpha mRNA. These data support the critical role of the activation of NF-kappaB in nitrite reductase-induced IL-8 gene expression in airway epithelium.
|
| 巻・号 |
67(8)
|
| ページ |
3872-8
|
| 公開日 |
1999-8-1
|
| DOI |
10.1128/IAI.67.8.3872-3878.1999
|
| PMID |
10417151
|
| PMC |
PMC96667
|
| MeSH |
DNA-Binding Proteins / metabolism
Epithelial Cells / metabolism
Gene Expression Regulation / drug effects*
Humans
I-kappa B Proteins*
Interleukin-8 / genetics*
Lung / metabolism*
NF-KappaB Inhibitor alpha
NF-kappa B / physiology*
Nitrite Reductases / pharmacology*
Promoter Regions, Genetic
Pseudomonas aeruginosa / enzymology*
Transcription Factor AP-1 / physiology
Tumor Cells, Cultured
|
| IF |
3.201
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098) |
