Reference - Detail
| RRC ID | 71659 |
|---|---|
| Author | Sugimoto M, Tada Y, Shichino S, Koyamatsu S, Tsumaki N, Abe K. |
| Title | Universal Surface Biotinylation: a simple, versatile and cost-effective sample multiplexing method for single-cell RNA-seq analysis. |
| Journal | DNA Res |
| Abstract |
Recent advances in single-cell analysis technology have made it possible to analyze tens of thousands of cells at a time. In addition, sample multiplexing techniques, which allow the analysis of several types of samples in a single run, are very useful for reducing experimental costs and improving experimental accuracy. However, a problem with this technique is that antigens and antibodies for universal labeling of various cell types may not be fully available. To overcome this issue, we developed a universal labeling technique, Universal Surface Biotinylation (USB), which does not depend on specific cell surface proteins. By introducing biotin into the amine group of any cell surface protein, we have obtained good labeling results in all the cell types we have tested. Combining with DNA-tagged streptavidin, it is possible to label each cell sample with specific DNA "hashtag". Compared with the conventional cell hashing method, the USB procedure seemed to have no discernible adverse effect on the acquisition of the transcriptome in each cell, according to the model experiments using differentiating mouse embryonic stem cells. This method can be theoretically used for any type of cells, including cells to which the conventional cell hashing method has not been applied successfully. |
| Volume | 29(3) |
| Published | 2022-5-27 |
| DOI | 10.1093/dnares/dsac017 |
| PII | 6598800 |
| PMID | 35652718 |
| PMC | PMC9202638 |
| MeSH | Animals Biotin* Biotinylation Cost-Benefit Analysis Mice Sequence Analysis, RNA Streptavidin |
| IF | 4.009 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | EB3(AES0139) |