論文 - 詳細
| RRC ID | 7165 |
|---|---|
| 著者 | Agawa Y, Sarhan M, Kageyama Y, Akagi K, Takai M, Hashiyama K, Wada T, Handa H, Iwamatsu A, Hirose S, Ueda H. |
| タイトル | Drosophila Blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway. |
| ジャーナル | Mol Cell Biol |
| Abstract |
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-f1 is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-f1. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway. |
| 巻・号 | 27(24) |
| ページ | 8739-47 |
| 公開日 | 2007-12-1 |
| DOI | 10.1128/MCB.01304-07 |
| PII | MCB.01304-07 |
| PMID | 17923694 |
| PMC | PMC2169387 |
| MeSH | Animals Binding Sites Cell Extracts Cell Nucleus / drug effects Cell Nucleus / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila Proteins / genetics Drosophila Proteins / isolation & purification Drosophila Proteins / metabolism* Drosophila melanogaster / drug effects* Drosophila melanogaster / embryology* Ecdysone / pharmacology* Embryo, Nonmammalian / drug effects Embryo, Nonmammalian / metabolism Gene Expression Regulation, Developmental / drug effects Promoter Regions, Genetic / genetics Pupa / drug effects RNA, Messenger / genetics RNA, Messenger / metabolism Repressor Proteins / genetics Repressor Proteins / isolation & purification Repressor Proteins / metabolism* Sequence Homology, Amino Acid Thermodynamics Time Factors Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic* / drug effects |
| IF | 3.611 |
| 引用数 | 36 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | hs-Gal4 lines |